Robin A. Felder

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Primary Appointment

Professor, Pathology

Education

  • PhD, Georgetown University

Research Disciplines

Biochemistry, Bioinformatics and Genomics, Cardiovascular Biology, Computational Biology, Epigenetics, Experimental Pathology, Genetics, Molecular Biology, Molecular Pharmacology, Structural Biology, Translational Science

Research Interests

Clinical Chemistry and Toxicology. Medical Automation Research. Neurotransmitters, cell surface receptors and intracellular second messengers.

Research Description

They have no symptoms, but subjects with untreated hypertension and salt sensitivity will have major health challenges such as blindness, stroke, kidney failure, and heart attacks. These devastating (and costly) endpoints will affect over 50% of the adult population. We have discovered a key regulatory pathway that controls sodium handling by epithelial cells, induces high blood pressure in mice, and is recapitulated in human test subjects. We have three laboratories (including support core facilities) focused on studying various aspects of the molecular basis of sodium excretion and blood pressure control. We employ genetic screening, cell based assays, clinical trial protocols, and behavioral surveys in order to fully understand the etiology of disease. Our automated drug discovery laboratory will also train students in modern techniques of setting up and running laboratory automation. In addition, we have a translational component of our research that has resulted in 8 "spin-out" companies, which have provided private sector opportunities for students post graduation.

Personal Statement

They have no symptoms, but subjects with untreated hypertension and salt sensitivity will have major health challenges such as blindness, stroke, kidney failure, and heart attacks. These devastating (and costly) endpoints will affect over 50% of the adult population. We have discovered key regulatory pathways that controls sodium handling by epithelial cells, induces high blood pressure in mice, and is recapitulated in human test subjects. We have three laboratories (including support core facilities) focused on studying various aspects of the molecular basis of sodium excretion and blood pressure control. We employ genetic screening, cell based assays, clinical trial protocols, and behavioral surveys in order to fully understand the etiology of disease. Our automated drug discovery laboratory will also train students in modern techniques of setting up and running laboratory automation. In addition, we have a translational component of our research that has resulted in 8 "spin-out" companies, which have provided private sector opportunities for students post graduation.

Selected Publications

Sanada H, Yoneda M, Yatabe J, Williams SM, Bartlett J, White MJ, Gordon LN, Felder RA, Eisner GM, Armando I, Jose PA, Common variants of the G protein-coupled receptor type 4 are associated with human essential hypertension and predict the blood pressure response to angiotensin receptor blockade., 2015; The pharmacogenomics journal. () . PMID: 25732908

Han F, Konkalmatt P, Chen J, Gildea J, Felder RA, Jose PA, Armando I, MiR-217 mediates the protective effects of the dopamine D2 receptor on fibrosis in human renal proximal tubule cells., 2015; Hypertension. 65(5) 1118-25. PMID: 25801876 | PMCID: PMC4393363

Li F, Yang J, Jones JE, Villar VA, Yu P, Armando I, Felder RA, Jose PA, Sorting nexin 5 and dopamine d1 receptor regulate the expression of the insulin receptor in human renal proximal tubule cells., 2015; Endocrinology. 156(6) 2211-21. PMID: 25825816 | PMCID: PMC4430625

Kitada K, Nakano D, Ohsaki H, Hitomi H, Minamino T, Yatabe J, Felder RA, Mori H, Masaki T, Kobori H, Nishiyama A, Hyperglycemia causes cellular senescence via a SGLT2- and p21-dependent pathway in proximal tubules in the early stage of diabetic nephropathy., 2014; Journal of diabetes and its complications. 28(5) 604-11. PMID: 24996978 | PMCID: PMC4153757

Felder RA, Automated specimen inspection, quality analysis, and its impact on patient safety: beyond the bar code., 2014; Clinical chemistry. 60(3) 433-4. PMID: 24407911

Armando I, Konkalmatt P, Felder RA, Jose PA, The renal dopaminergic system: novel diagnostic and therapeutic approaches in hypertension and kidney disease., 2014; Translational research : the journal of laboratory and clinical medicine. 165(4) 505-11. PMID: 25134060 | PMCID: PMC4305499

Yokokawa H, Goto A, Sanada H, Watanabe T, Felder RA, Jose PA, Yasumura S, Association between control to target blood pressures and healthy lifestyle factors among Japanese hypertensive patients: longitudinal data analysis from Fukushima Research of Hypertension (FRESH)., 2014; Obesity research & clinical practice. 8(4) e364-73. PMID: 25091358

Yu P, Sun M, Villar VA, Zhang Y, Weinman EJ, Felder RA, Jose PA, Differential dopamine receptor subtype regulation of adenylyl cyclases in lipid rafts in human embryonic kidney and renal proximal tubule cells., 2014; Cellular signalling. 26(11) 2521-9. PMID: 25049074 | PMCID: PMC4166567

Gildea JJ, Seaton JE, Victor KG, Reyes CM, Bigler Wang D, Pettigrew AC, Courtner CE, Shah N, Tran HT, Van Sciver RE, Carlson JM, Felder RA, Exosomal transfer from human renal proximal tubule cells to distal tubule and collecting duct cells., 2014; Clinical biochemistry. 47(15) 89-94. PMID: 24976626

Felder RA, Jackson KD, Walter AM, Process evaluation of an open architecture real-time molecular laboratory platform., 2014; Journal of laboratory automation. 19(5) 468-73. PMID: 24811476

Sanada H, Yokokawa H, Yatabe J, Williams SM, Felder RA, Jose PA, Takenosita S, Association between lifestyle-related disorders and visceral fat mass in Japanese males: a hospital based cross-sectional study., 2014; Environmental health and preventive medicine. 19(6) 429-35. PMID: 25248615 | PMCID: PMC4235847

Yu P, Han W, Villar VA, Yang Y, Lu Q, Lee H, Li F, Quinn MT, Gildea JJ, Felder RA, Jose PA, Unique role of NADPH oxidase 5 in oxidative stress in human renal proximal tubule cells., 2014; Redox biology. 2() 570-9. PMID: 24688893 | PMCID: PMC3969603

Gildea JJ, Shah IT, Van Sciver RE, Israel JA, Enzensperger C, McGrath HE, Jose PA, Felder RA, The cooperative roles of the dopamine receptors, D1R and D5R, on the regulation of renal sodium transport., 2014; Kidney international. 86(1) 118-26. PMID: 24552847 | PMCID: PMC4077925

Jiang X, Konkalmatt P, Yang Y, Gildea J, Jones JE, Cuevas S, Felder RA, Jose PA, Armando I, Single-nucleotide polymorphisms of the dopamine D2 receptor increase inflammation and fibrosis in human renal proximal tubule cells., 2014; Hypertension. 63(3) e74-80. PMID: 24379187 | PMCID: PMC3929206

Lee H, Abe Y, Lee I, Shrivastav S, Crusan AP, Hüttemann M, Hopfer U, Felder RA, Asico LD, Armando I, Jose PA, Kopp JB, Increased mitochondrial activity in renal proximal tubule cells from young spontaneously hypertensive rats., 2013; Kidney international. () . PMID: 24132210 | PMCID: NIHMS521548

Armando I, Villar VA, Jones JE, Lee H, Wang X, Asico LD, Yu P, Yang J, Escano CS, Pascua-Crusan AM, Felder RA, Jose PA, Dopamine D3 receptor inhibits the ubiquitin-specific peptidase 48 to promote NHE3 degradation., 2013; FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 28(3) 1422-34. PMID: 24308971 | PMCID: PMC3929671

Gildea JJ, Lahiff DT, Van Sciver RE, Weiss RS, Shah N, McGrath HE, Schoeffel CD, Jose PA, Carey RM, Felder RA, A linear relationship between the ex-vivo sodium mediated expression of two sodium regulatory pathways as a surrogate marker of salt sensitivity of blood pressure in exfoliated human renal proximal tubule cells: the virtual renal biopsy., 2013; Clinica chimica acta; international journal of clinical chemistry. 421() 236-42. PMID: 23454474 | PMCID: PMC3724455

Gildea JJ, Tran HT, Van Sciver RE, Bigler Wang D, Carlson JM, Felder RA, A novel role for c-Myc in G protein-coupled receptor kinase 4 (GRK4) transcriptional regulation in human kidney proximal tubule cells., 2013; Hypertension. 61(5) 1021-7. PMID: 23509080 | PMCID: PMC3640476

Gildea JJ, Carlson JM, Schoeffel CD, Carey RM, Felder RA, Urinary exosome miRNome analysis and its applications to salt sensitivity of blood pressure., 2013; Clinical biochemistry. 46(12) 1131-4. PMID: 23726803 | PMCID: PMC3786136

Gildea JJ, McGrath HE, Van Sciver RE, Wang DB, Felder RA, Isolation, growth, and characterization of human renal epithelial cells using traditional and 3D methods., 2012; Methods in molecular biology (Clifton, N.J.). 945() 329-45. PMID: 23097116

Wei SG, Zhang ZH, Yu Y, Weiss RM, Felder RB, Central actions of the chemokine stromal cell-derived factor 1 contribute to neurohumoral excitation in heart failure rats., 2012; Hypertension. 59(5) 991-8. PMID: 22493069 | PMCID: PMC3366637

Yatabe J, Yatabe MS, Yoneda M, Felder RA, Jose PA, Sanada H, Hypertension-Related Gene Polymorphisms of G-Protein-Coupled Receptor Kinase 4 Are Associated with NT-proBNP Concentration in Normotensive Healthy Adults., 2012; International journal of hypertension. 2012() 806810. PMID: 22518293 | PMCID: PMC3296214

Yang Y, Zhang Y, Cuevas S, Villar VA, Escano C, D Asico L, Yu P, Grandy DK, Felder RA, Armando I, Jose PA, Paraoxonase 2 decreases renal reactive oxygen species production, lowers blood pressure, and mediates dopamine D2 receptor-induced inhibition of NADPH oxidase., 2012; Free radical biology & medicine. 53(3) 437-46. PMID: 22634053 | PMCID: PMC3408834

Locke C, McEuen J, Felder RS, Lesselroth B, Barrett TW, Stevens B, Pope DS, Evaluating an insulin infusion protocol in an acute care setting., 2012; Clinical nurse specialist CNS. 25(5) 237-43. PMID: 22649844

Gildea JJ, Wang X, Shah N, Tran H, Spinosa M, Van Sciver R, Sasaki M, Yatabe J, Carey RM, Jose PA, Felder RA, Dopamine and angiotensin type 2 receptors cooperatively inhibit sodium transport in human renal proximal tubule cells., 2012; Hypertension. 60(2) 396-403. PMID: 22710646 | PMCID: PMC3406928

Sanada H, Yokokawa H, Yoneda M, Yatabe J, Sasaki Yatabe M, Williams SM, Felder RA, Jose PA, High body mass index is an important risk factor for the development of type 2 diabetes., 2012; Internal medicine (Tokyo, Japan). 51(14) 1821-6. PMID: 22821094 | PMCID: PMC3540801

Carey RM, Schoeffel CD, Gildea JJ, Jones JE, McGrath HE, Gordon LN, Park MJ, Sobota RS, Underwood PC, Williams J, Sun B, Raby B, Lasky-Su J, Hopkins PN, Adler GK, Williams SM, Jose PA, Felder RA, Salt sensitivity of blood pressure is associated with polymorphisms in the sodium-bicarbonate cotransporter., 2012; Hypertension. 60(5) 1359-66. PMID: 22987918 | PMCID: PMC3495588

Gudmundsson H, Curran J, Kashef F, Snyder JS, Smith SA, Vargas-Pinto P, Bonilla IM, Weiss RM, Anderson ME, Binkley P, Felder RB, Carnes CA, Band H, Hund TJ, Mohler PJ, Differential regulation of EHD3 in human and mammalian heart failure., 2012; Journal of molecular and cellular cardiology. 52(5) 1183-90. PMID: 22406195 | PMCID: PMC3360944

Villar VA, Jones JE, Armando I, Asico LD, Escano CS, Lee H, Wang X, Yang Y, Pascua-Crusan AM, Palmes-Saloma CP, Felder RA, Jose PA, Sorting nexin 1 loss results in d5 dopamine receptor dysfunction in human renal proximal tubule cells and hypertension in mice., 2012; The Journal of biological chemistry. 288(1) 152-63. PMID: 23152498 | PMCID: PMC3537009

Felder RA, White MJ, Williams SM, Jose PA, Diagnostic tools for hypertension and salt sensitivity testing., 2012; Current opinion in nephrology and hypertension. 22(1) 65-76. PMID: 23197156

Yokokawa H, Goto A, Sanada H, Watanabe T, Felder RA, Jose PA, Yasumura S, Achievement status toward goal blood pressure levels and healthy lifestyles among Japanese hypertensive patients; cross-sectional survey results from Fukushima Research of Hypertension (FRESH)., 2011; Internal medicine (Tokyo, Japan). 50(11) 1149-56. PMID: 21628928

Zhang ZH, Yu Y, Wei SG, Felder RB, Aldosterone-induced brain MAPK signaling and sympathetic excitation are angiotensin II type-1 receptor dependent., 2011; American journal of physiology. Heart and circulatory physiology. 302(3) H742-51. PMID: 22081704 | PMCID: PMC3353800

Zhang ZH, Yu Y, Wei SG, Nakamura Y, Nakamura K, Felder RB, EPâ receptors mediate PGEâ-induced hypothalamic paraventricular nucleus excitation and sympathetic activation., 2011; American journal of physiology. Heart and circulatory physiology. 301(4) H1559-69. PMID: 21803943 | PMCID: PMC3197370

Yu Y, Zhang ZH, Wei SG, Weiss RM, Felder RB, Peroxisome proliferator-activated receptor-γ regulates inflammation and renin-angiotensin system activity in the hypothalamic paraventricular nucleus and ameliorates peripheral manifestations of heart failure., 2011; Hypertension. 59(2) 477-84. PMID: 22083161 | PMCID: PMC3266457

Yatabe J, Yoneda M, Yatabe MS, Watanabe T, Felder RA, Jose PA, Sanada H, Angiotensin III stimulates aldosterone secretion from adrenal gland partially via angiotensin II type 2 receptor but not angiotensin II type 1 receptor., 2011; Endocrinology. 152(4) 1582-8. PMID: 21303953

Gildea JJ, Kemp BA, Howell NL, Van Sciver RE, Carey RM, Felder RA, Inhibition of renal caveolin-1 reduces natriuresis and produces hypertension in sodium-loaded rats., 2011; American journal of physiology. Renal physiology. 300(4) F914-20. PMID: 21289050 | PMCID: PMC3074998

Yu P, Han W, Villar VA, Li H, Arnaldo FB, Concepcion GP, Felder RA, Quinn MT, Jose PA, Dopamine D1 receptor-mediated inhibition of NADPH oxidase activity in human kidney cells occurs via protein kinase A-protein kinase C cross talk., 2011; Free radical biology & medicine. 50(7) 832-40. PMID: 21193028 | PMCID: PMC3066436

Felder RA, Preanalytical errors introduced by sample-transportation systems: a means to assess them., 2011; Clinical chemistry. 57(10) 1349-50. PMID: 21836080