Thurl E Harris

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Primary Appointment

Associate Professor and Director of Graduate Studies, Pharmacology

Education

  • PhD, Cell & Molecular Biology, Baylor College of Medicine

Research Disciplines

Biochemistry, Biology, Biotechnology, Cancer Biology, Cardiovascular Biology, Kinesiology, Metabolism, Molecular and Cellular Physiology, Molecular Biology, Molecular Pharmacology, Physiology, Translational Science

Research Interests

Molecular mechanisms controlling insulin signaling and fat synthesis.

Research Description

As Paleolithic hunter gatherers, the human diet was relatively low in fats and simple carbohydrates. Technological advances, beginning with the agricultural revolution 12,000 years ago and culminating with the mechanization of food production in the last century, have provided a significant portion of the worldâs population with access to inexpensive, energy-dense food. In the modern age the ready availability of carbohydrates and fats, combined with a large reduction in physical labor, has led to a predictable rise in obesity. The Center for Disease Control estimates currently 34% of the U.S. population is characterized as overweight (B.M.I. of 25-30), and another 41% are obese (B.M.I.>30), giving an astonishing 3 out 4 Americans defined as overweight or obese. As an underlying risk factor for type II diabetes and cardiovascular disease, understanding how obesity promotes the development of these diseases is of extreme importance for worldwide health.
Food intake triggers the release of insulin, an anabolic hormone that promotes nutrient storage and protein synthesis. Our laboratory is interested in the mechanisms underlying the storage and release of carbohydrates and fats as triacylglycerol (TAG) in adipose tissue, and the dysregulation of insulin signaling that occurs during obesity.
The mTOR Signaling Pathway - A key player in the insulin signaling cascade is the mammalian target of rapamycin (mTOR), a highly conserved Ser-Thr phosphatidylinositol 3-kinase-related protein kinase that integrates cellular energy status and growth factors to regulate cellular growth, survival, and metabolism. mTOR forms two distinct complexes, mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2) each of which catalyzes the phosphorylation of different substrates in response to insulin. Under conditions of caloric excess, such as that seen during diet-induced obesity, both arms of the mTOR pathway can be affected. We study the molecular mechanisms of how insulin signaling activates the two different mTOR complexes, the targets of mTORC1 and 2, and the functional consequences of obesity on mTOR activity.
Triacylglycerol Storage in Adipocytes and Inflammation - Another important research interest in the laboratory are the enzymatic pathways by which adipocytes store fatty acids as TAG and release fatty acids for energy between meals. It is clear that obesity results in dysregulation of the storage and breakdown of TAG in adipose tissue and genetic studies in mice have suggested that improper regulation of this process can cause peripheral insulin resistance and glucose intolerance- both hallmarks of the pre-diabetes. We study the molecular alterations in TAG homeostasis in adipocytes and how this can impact the physiology of whole body metabolism.

Personal Statement

My primary research goal is to identify alterations that occur in the insulin signaling pathway during diabetes and obesity. Most recently I have focused on the role of the mTOR signaling pathway, and one of its downstream targets, the phosphatidic acid phosphatase lipin 1, in insulin responsive tissues such as adipose and skeletal muscle. To this end my laboratory has developed techniques for measuring lipogenesis and lipolysis in insulin responsive cells, tissues, and whole animals. The methods include comprehensive LC-MS methods for quantitating glycerolipid intermediates generated during the buildup or breakdown of triacylglycerol. My laboratory performs a comprehensive range of experimental studies ranging from whole animal glucose metabolism to transcriptional analysis, from the molecular mechanisms controlling kinase signaling cascades to lipid synthesis.

Training

  • Basic Cardiovascular Research Training Grant
  • Biotechnology Training Grant
  • Training in Cell and Molecular Biology
  • Training in the Pharmacological Sciences

Selected Publications

2024

Sharifian Gh, M., Norouzi, F., Sorci, M., Zaid, T. S., Pier, G. B., Achimovich, A., . . . Laurie, G. W. (2024). Targeting Iron - Respiratory Reciprocity Promotes Bacterial Death.. bioRxiv. doi:10.1101/2024.03.01.582947

2023

Beretta, M., Dai, Y., Olzomer, E. M., Vancuylenburg, C. S., Santiago-Rivera, J. A., Philp, A. M., . . . Hoehn, K. L. (2024). Liver-Selective Imidazolopyrazine Mitochondrial Uncoupler SHD865 Reverses Adiposity and Glucose Intolerance in Mice. DIABETES, 73(3), 374-384. doi:10.2337/db23-0233

Heindel, A. J., Brulet, J. W., Wang, X., Founds, M. W., Libby, A. H., Bai, D. L., . . . Hsu, K. -L. (2023). Chemoproteomic capture of RNA binding activity in living cells. NATURE COMMUNICATIONS, 14(1). doi:10.1038/s41467-023-41844-z

Mendez, R., Shaikh, M., Lemke, M. C., Yuan, K., Libby, A. H., Bai, D. L., . . . Hsu, K. -L. (2023). Predicting small molecule binding pockets on diacylglycerol kinases using chemoproteomics and AlphaFold. RSC CHEMICAL BIOLOGY, 4(6), 422-430. doi:10.1039/d3cb00057e

Murray, J. H., Burgio, A. L., Beretta, M., Hargett, S. R., Harris, T. E., Olzomer, E., . . . Santos, W. L. (2023). Oxadiazolopyridine Derivatives as Efficacious Mitochondrial Uncouplers in the Prevention of Diet-Induced Obesity. JOURNAL OF MEDICINAL CHEMISTRY, 66(6), 3876-3895. doi:10.1021/acs.jmedchem.2c01573

2022

Yeudall, S., Upchurch, C. M., Seegren, P. V., Pavelec, C. M., Greulich, J., Lemke, M. C., . . . Leitinger, N. (2022). Macrophage acetyl-CoA carboxylase regulates acute inflammation through control of glucose and lipid metabolism. SCIENCE ADVANCES, 8(47). doi:10.1126/sciadv.abq1984

Bottermann, K., Kalfhues, L., Nederlof, R., Hemmers, A., Leitner, L. M., Oenarto, V., . . . Goedecke, A. (2022). Cardiomyocyte p38 MAPKα suppresses a heart-adipose tissue-neutrophil crosstalk in heart failure development. BASIC RESEARCH IN CARDIOLOGY, 117(1). doi:10.1007/s00395-022-00955-2

Granade, M. E., Hargett, S. R., Lank, D. S., Lemke, M. C., Luse, M. A., Isakson, B. E., . . . Harris, T. E. (2022). Feeding desensitizes A1 adenosine receptors in adipose through FOXO1-mediated transcriptional regulation. MOLECULAR METABOLISM, 63. doi:10.1016/j.molmet.2022.101543

Yang, Q., Hinkle, J., Reed, J. N., Aherrahrou, R., Xu, Z., Harris, T. E., . . . Civelek, M. (2022). Adipocyte-Specific Modulation of KLF14 Expression in Mice Leads to Sex-Dependent Impacts on Adiposity and Lipid Metabolism. DIABETES, 71(4), 677-693. doi:10.2337/db21-0674

Granade, M. E., Manigat, L. C., Lemke, M. C., Purow, B. W., & Harris, T. E. (2022). Identification of ritanserin analogs that display DGK isoform specificity. BIOCHEMICAL PHARMACOLOGY, 197. doi:10.1016/j.bcp.2022.114908

2021

Kaur, S., Auger, C., Barayan, D., Shah, P., Matveev, A., Knuth, C. M., . . . Jeschke, M. G. (2021). Adipose-specific ATGL ablation reduces burn injury-induced metabolic derangements in mice. CLINICAL AND TRANSLATIONAL MEDICINE, 11(6). doi:10.1002/ctm2.417

Huang, T., Hosseinibarkooie, S., Borne, A. L., Granade, M. E., Brulet, J. W., Harris, T. E., . . . Hsu, K. -L. (2021). Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. CHEMICAL SCIENCE, 12(9), 3295-3307. doi:10.1039/d0sc06623k

Merta, H., Rodriguez, J. W. C., Anjur-Dietrich, M. I., Vitale, T., Granade, M. E., Harris, T. E., . . . Bahmanyar, S. (2021). Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregation. DEVELOPMENTAL CELL, 56(24), 3364-+. doi:10.1016/j.devcel.2021.11.009

Manigat, L. C., Granade, M. E., Taori, S., Miller, C. A., Vass, L. R., Zhong, X. -P., . . . Purow, B. W. (2021). Loss of Diacylglycerol Kinase α Enhances Macrophage Responsiveness. FRONTIERS IN IMMUNOLOGY, 12. doi:10.3389/fimmu.2021.722469

Wang, H., Chan, T. W., Vashisht, A. A., Drew, B. G., Calkin, A. C., Harris, T. E., . . . Reue, K. (2021). Lipin 1 modulates mRNA splicing during fasting adaptation in liver. JCI INSIGHT, 6(17). doi:10.1172/jci.insight.150114

Kearns, S. M., Ahern, K. W., Patrie, J. T., Horton, W. B., Harris, T. E., & Kadl, A. (2021). Reduced adiponectin levels in patients with COVID-19 acute respiratory failure: A case-control study. PHYSIOLOGICAL REPORTS, 9(7). doi:10.14814/phy2.14843

Bottermann, K., Granade, M. E., Oenarto, V., Fischer, J. W., & Harris, T. E. (2021). Atglistatin Pretreatment Preserves Remote Myocardium Function Following Myocardial Infarction. JOURNAL OF CARDIOVASCULAR PHARMACOLOGY AND THERAPEUTICS, 26(3), 289-297. doi:10.1177/1074248420971113

2020

Senthivinayagam, S., Serbulea, V., Upchurch, C. M., Polanowska-Grabowska, R., Mendu, S. K., Sahu, S., . . . Leitinger, N. (2021). Adaptive thermogenesis in brown adipose tissue involves activation of pannexin-1 channels. MOLECULAR METABOLISM, 44. doi:10.1016/j.molmet.2020.101130

Ware, T. B., Franks, C. E., Granade, M. E., Zhang, M., Kim, K. -B., Park, K. -S., . . . Hsu, K. -L. (2020). Reprogramming fatty acyl specificity of lipid kinases via C1 domain engineering. NATURE CHEMICAL BIOLOGY, 16(2), 170-+. doi:10.1038/s41589-019-0445-9

Martinez, B. A., Hoyle, R. G., Yeudall, S., Granade, M. E., Harris, T. E., Castle, J. D., . . . Bland, M. L. (2020). Innate immune signaling in Drosophila shifts anabolic lipid metabolism from triglyceride storage to phospholipid synthesis to support immune function. PLOS GENETICS, 16(11). doi:10.1371/journal.pgen.1009192

Raje, V., Ahern, K. W., Martinez, B. A., Howell, N. L., Oenarto, V., Granade, M. E., . . . Harris, T. E. (2020). Adipocyte lipolysis drives acute stress-induced insulin resistance. SCIENTIFIC REPORTS, 10(1). doi:10.1038/s41598-020-75321-0

Kharel, Y., Huang, T., Salamon, A., Harris, T. E., Santos, W. L., & Lynch, K. R. (2020). Mechanism of sphingosine 1-phosphate clearance from blood. BIOCHEMICAL JOURNAL, 477(5), 925-935. doi:10.1042/BCJ20190730

Kruger, N., Biwer, L. A., Good, M. E., Ruddiman, C. A., Wolpe, A. G., DeLalio, L. J., . . . Isakson, B. E. (2020). Loss of Endothelial FTO Antagonizes Obesity-Induced Metabolic and Vascular Dysfunction. CIRCULATION RESEARCH, 126(2), 232-242. doi:10.1161/CIRCRESAHA.119.315531

2019

Ahern, K. W., Serbulea, V., Wingrove, C. L., Palas, Z. T., Leitinger, N., & Harris, T. E. (2019). Regioisomer-independent quantification of fatty acid oxidation products by HPLC-ESI-MS/MS analysis of sodium adducts. SCIENTIFIC REPORTS, 9. doi:10.1038/s41598-019-47693-5

Hennessy, M., Granade, M. E., Hassaninasab, A., Wang, D., Kwiatek, J. M., Han, G. -S., . . . Carman, G. M. (2019). Casein kinase II-mediated phosphorylation of lipin 1β phosphatidate phosphatase at Ser-285 and Ser-287 regulates its interaction with 14-3-3β protein. JOURNAL OF BIOLOGICAL CHEMISTRY, 294(7), 2365-2374. doi:10.1074/jbc.RA118.007246

Schweitzer, G. G., Collier, S. L., Chen, Z., McCommis, K. S., Pittman, S. K., Yoshino, J., . . . Finck, B. N. (2019). Loss of lipin 1-mediated phosphatidic acid phosphohydrolase activity in muscle leads to skeletal myopathy in mice. FASEB JOURNAL, 33(1), 652-667. doi:10.1096/fj.201800361R

2018

Cantwell, M. T., Farrar, J. S., Lownik, J. C., Meier, J. A., Hyun, M., Raje, V., . . . Larner, A. C. (2018). STAT3 suppresses Wnt/β-catenin signaling during the induction phase of primary Myf5+brown adipogenesis. CYTOKINE, 111, 434-444. doi:10.1016/j.cyto.2018.05.023

Granade, M. E., & Harris, T. E. (2018). Purification of Lipin and Measurement of Phosphatidic Acid Phosphatase Activity from Liposomes. PHOSPHATASES, 607, 373-388. doi:10.1016/bs.mie.2018.04.028

Haskins, R. M., Nguyen, A. T., Alencar, G. F., Billaud, M., Kelly-Goss, M. R., Good, M. E., . . . Owens, G. K. (2018). Klf4 has an unexpected protective role in perivascular cells within the microvasculature. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 315(2), H402-H414. doi:10.1152/ajpheart.00084.2018

Tong, Y., Park, S., Wu, D., Harris, T. E., Moskaluk, C. A., Brautigan, D. L., & Fu, Z. (2018). Modulation of GSK3β autoinhibition by Thr-7 and Thr-8. FEBS LETTERS, 592(4), 537-546. doi:10.1002/1873-3468.12990

Serbulea, V., Upchurch, C. M., Ahern, K. W., Bories, G., Voigt, P., DeWeese, D. E., . . . Leitinger, N. (2018). Macrophages sensing oxidized DAMPs reprogram their metabolism to support redox homeostasis and inflammation through a TLR2-Syk-ceramide dependent mechanism. MOLECULAR METABOLISM, 7, 23-34. doi:10.1016/j.molmet.2017.11.002

Olmez, I., Love, S., Xiao, A., Manigat, L., Randolph, P., McKenna, B. D., . . . Purow, B. (2018). Targeting the mesenchymal subtype in glioblastoma and other cancers via inhibition of diacylglycerol kinase alpha. NEURO-ONCOLOGY, 20(2), 192-202. doi:10.1093/neuonc/nox119

2017

McCloud, R. L., Franks, C. E., Campbell, S. T., Purow, B. W., Harris, T. E., & Hsu, K. -L. (2018). Deconstructing Lipid Kinase Inhibitors by Chemical Proteomics. BIOCHEMISTRY, 57(2), 231-236. doi:10.1021/acs.biochem.7b00962

Yang, C. -S., Melhuish, T. A., Spencer, A., Ni, L., Hao, Y., Jividen, K., . . . Paschal, B. M. (2017). The protein kinase C super-family member PKN is regulated by mTOR and influences differentiation during prostate cancer progression. PROSTATE, 77(15), 1452-1467. doi:10.1002/pros.23400

Boroda, S., Takkellapati, S., Lawrence, R. T., Entwisle, S. W., Pearson, J. M., Granade, M. E., . . . Harris, T. E. (2017). The phosphatidic acid-binding, polybasic domain is responsible for the differences in the phosphoregulation of lipins 1 and 3. JOURNAL OF BIOLOGICAL CHEMISTRY, 292(50), 20481-20493. doi:10.1074/jbc.M117.786574

Franks, C. E., Campbell, S. T., Purow, B. W., Harris, T. E., & Hsu, K. -L. (2017). The Ligand Binding Landscape of Diacylglycerol Kinases. CELL CHEMICAL BIOLOGY, 24(7), 870-+. doi:10.1016/j.chembiol.2017.06.007

He, J., Zhang, F., Tay, L. W. R., Boroda, S., Nian, W., Levental, K. R., . . . Du, G. (2017). Lipin-1 regulation of phospholipid synthesis maintains endoplasmic reticulum homeostasis and is critical for triple-negative breast cancer cell survival. FASEB JOURNAL, 31(7), 2893-2904. doi:10.1096/fj.201601353R

Gaykema, R. P., Newmyer, B. A., Ottolini, M., Raje, V., Warthen, D. M., Lambeth, P. S., . . . Scott, M. M. (2017). Activation of murine pre-proglucagon-producing neurons reduces food intake and body weight. JOURNAL OF CLINICAL INVESTIGATION, 127(3), 1031-1045. doi:10.1172/JCI81335

Taddeo, E. P., Hargett, S. R., Lahiri, S., Nelson, M. E., Liao, J. A., Li, C., . . . Hoehn, K. L. (2017). Lysophosphatidic acid counteracts glucagon-induced hepatocyte glucose production via STAT3. SCIENTIFIC REPORTS, 7. doi:10.1038/s41598-017-00210-y

Boroda, S., Niccum, M., Raje, V., Purow, B. W., & Harris, T. E. (2017). Dual activities of ritanserin and R59022 as DGKα inhibitors and serotonin receptor antagonists. BIOCHEMICAL PHARMACOLOGY, 123, 29-39. doi:10.1016/j.bcp.2016.10.011

Raje, V., Derecka, M., Cantwell, M., Meier, J., Szczepanek, K., Sisler, J. D., . . . Larner, A. C. (2017). Kinase Inactive Tyrosine Kinase (Tyk2) Supports Differentiation of Brown Fat Cells. ENDOCRINOLOGY, 158(1), 148-157. doi:10.1210/en.2015-2048

2016

Pagac, M., Cooper, D. E., Qi, Y., Lukmantara, I. E., Mak, H. Y., Wu, Z., . . . Yang, H. (2016). SEIPIN Regulates Lipid Droplet Expansion and Adipocyte Development by Modulating the Activity of Glycerol-3-phosphate Acyltransferase. CELL REPORTS, 17(6), 1546-1559. doi:10.1016/j.celrep.2016.10.037

Boroda, S., Niccum, M., Raje, V., Purow, B. W., & Harris, T. E. (2017). Dual activities of ritanserin and R59022 as DGKα inhibitors and serotonin receptor antagonists.. Biochemical pharmacology, 123, 29-39. doi:10.1016/j.bcp.2016.10.011

Adamson, S. E., Griffiths, R., Moravec, R., Senthivinayagam, S., Montgomery, G., Chen, W., . . . Leitinger, N. (2016). Disabled homolog 2 controls macrophage phenotypic polarization and adipose tissue inflammation. JOURNAL OF CLINICAL INVESTIGATION, 126(4), 1311-1322. doi:10.1172/JCI79590

2015

Adamson, S. E., Meher, A. K., Chiu, Y. -H., Sandilos, J. K., Oberholtzer, N. P., Walker, N. N., . . . Leitinger, N. (2015). Pannexin 1 is required for full activation of insulin-stimulated glucose uptake in adipocytes. MOLECULAR METABOLISM, 4(9), 610-618. doi:10.1016/j.molmet.2015.06.009

Sisler, J. D., Morgan, M., Raje, V., Grande, R. C., Derecka, M., Meier, J., . . . Larner, A. C. (2015). The Signal Transducer and Activator of Transcription 1 (STAT1) Inhibits Mitochondrial Biogenesis in Liver and Fatty Acid Oxidation in Adipocytes. PLOS ONE, 10(12). doi:10.1371/journal.pone.0144444

Chao, H., Li, H., Grande, R., Lira, V., Yan, Z., Harris, T. E., & Li, C. (2015). Involvement of mTOR in Type 2 CRF Receptor Inhibition of Insulin Signaling in Muscle Cells. MOLECULAR ENDOCRINOLOGY, 29(6), 831-841. doi:10.1210/me.2014-1245

Zhang, C., Hwarng, G., Cooper, D. E., Grevengoed, T. J., Eaton, J. M., Natarajan, V., . . . Coleman, R. A. (2015). Inhibited Insulin Signaling in Mouse Hepatocytes Is Associated with Increased Phosphatidic Acid but Not Diacylglycerol. JOURNAL OF BIOLOGICAL CHEMISTRY, 290(6), 3519-3528. doi:10.1074/jbc.M114.602789

2014

Creutz, C. E., Eaton, J. M., & Harris, T. E. (2014). Correction to assembly of high molecular weight complexes of lipin on a supported lipid bilayer observed by atomic force microscopy.. Biochemistry, 53(49), 7866. doi:10.1021/bi501457g

Creutz, C. E., Eaton, J. M., & Harris, T. E. (2014). Assembly of High Molecular Weight Complexes of Lipin on a Supported Lipid Bilayer Observed by Atomic Force Microscopy (vol 52, pg 5092, 2013). BIOCHEMISTRY, 53(49), 7866. doi:10.1021/bi501457g

Mullins, G. R., Wang, L., Raje, V., Sherwood, S. G., Grande, R. C., Boroda, S., . . . Harris, T. E. (2014). Catecholamine-induced lipolysis causes mTOR complex dissociation and inhibits glucose uptake in adipocytes. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 111(49), 17450-17455. doi:10.1073/pnas.1410530111

Eaton, J. M., Takkellapati, S., Lawrence, R. T., McQueeney, K. E., Boroda, S., Mullins, G. R., . . . Harris, T. E. (2014). Lipin 2 Binds Phosphatidic Acid by the Electrostatic Hydrogen Bond Switch Mechanism Independent of Phosphorylation. JOURNAL OF BIOLOGICAL CHEMISTRY, 289(26), 18055-18066. doi:10.1074/jbc.M114.547604

Zhang, C., Cooper, D. E., Grevengoed, T. J., Li, L. O., Klett, E. L., Eaton, J. M., . . . Coleman, R. A. (2014). Glycerol-3-phosphate acyltransferase-4-deficient mice are protected from diet-induced insulin resistance by the enhanced association of mTOR and rictor. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 307(3), E305-E315. doi:10.1152/ajpendo.00034.2014

Kok, B. P. C., Skene-Arnold, T. D., Ling, J., Benesch, M. G. K., Dewald, J., Harris, T. E., . . . Brindley, D. N. (2014). Conserved Residues in the N Terminus of Lipin-1 Are Required for Binding to Protein Phosphatase-1c, Nuclear Translocation, and Phosphatidate Phosphatase Activity. JOURNAL OF BIOLOGICAL CHEMISTRY, 289(15), 10876-10886. doi:10.1074/jbc.M114.552612

Taddeo, E. P., Laker, R. C., Breen, D. S., Akhtar, Y. N., Kenwood, B. M., Liao, J. A., . . . Hoehn, K. L. (2014). Opening of the mitochondria! permeability transition pore links mitochondria! dysfunction to insulin resistance in skeletal muscle. MOLECULAR METABOLISM, 3(2), 124-134. doi:10.1016/j.molmet.2013.11.003

2013

Creutz, C. E., Eaton, J. M., & Harris, T. E. (2013). Assembly of High Molecular Weight Complexes of Lipin on a Supported Lipid Bilayer Observed by Atomic Force Microscopy. BIOCHEMISTRY, 52(30), 5092-5102. doi:10.1021/bi4004765

Kok, B. P. C., Dyck, J. R. B., Harris, T. E., & Brindley, D. N. (2013). Differential regulation of the expressions of the PGC-1α splice variants, lipins, and PPARα in heart compared to liver. JOURNAL OF LIPID RESEARCH, 54(6), 1662-1677. doi:10.1194/jlr.M036624

Eaton, J. M., Mullins, G. R., Brindley, D. N., & Harris, T. E. (2013). Phosphorylation of Lipin 1 and Charge on the Phosphatidic Acid Head Group Control Its Phosphatidic Acid Phosphatase Activity and Membrane Association. JOURNAL OF BIOLOGICAL CHEMISTRY, 288(14), 9933-9945. doi:10.1074/jbc.M112.441493

Mitra, M. S., Chen, Z., Ren, H., Harris, T. E., Chambers, K. T., Hall, A. M., . . . Finck, B. N. (2013). Mice with an adipocyte-specific lipin 1 separation-of-function allele reveal unexpected roles for phosphatidic acid in metabolic regulation. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 110(2), 642-647. doi:10.1073/pnas.1213493110

Dominguez, C. L., Floyd, D. H., Xiao, A., Mullins, G. R., Kefas, B. A., Xin, W., . . . Purow, B. W. (2013). Diacylglycerol Kinase a Is a Critical Signaling Node and Novel Therapeutic Target in Glioblastoma and Other Cancers. CANCER DISCOVERY, 3(7), 782-797. doi:10.1158/2159-8290.CD-12-0215

2012

Wu, D., Chapman, J. R., Wang, L., Harris, T. E., Shabanowitz, J., Hunt, D. F., & Fu, Z. (2012). Intestinal Cell Kinase (ICK) Promotes Activation of mTOR Complex 1 (mTORC1) through Phosphorylation of Raptor Thr-908. JOURNAL OF BIOLOGICAL CHEMISTRY, 287(15), 12510-12519. doi:10.1074/jbc.M111.302117

Zhang, C., Wendel, A. A., Keogh, M. R., Harris, T. E., Chen, J., & Coleman, R. A. (2012). Glycerolipid signals alter mTOR complex 2 (mTORC2) to diminish insulin signaling. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109(5), 1667-1672. doi:10.1073/pnas.1110730109

Harris, T. E., & Thorner, M. O. (2012). Caloric Restriction in mTORC1 Control of Intestinal Homeostasis. CELL METABOLISM, 16(1), 6-8. doi:10.1016/j.cmet.2012.06.010

Gonzalez, I. M., Martin, P. M., Burdsal, C., Sloan, J. L., Mager, S., Harris, T., & Sutherland, A. E. (2012). Leucine and arginine regulate trophoblast motility through mTOR-dependent and independent pathways in the preimplantation mouse embryo. DEVELOPMENTAL BIOLOGY, 361(2), 286-300. doi:10.1016/j.ydbio.2011.10.021

Glidden, E. J., Gray, L. G., Vemuru, S., Li, D., Harris, T. E., & Mayo, M. W. (2012). Multiple Site Acetylation of Rictor Stimulates Mammalian Target of Rapamycin Complex 2 (mTORC2)-dependent Phosphorylation of Akt Protein. JOURNAL OF BIOLOGICAL CHEMISTRY, 287(1), 581-588. doi:10.1074/jbc.M111.304337

Derecka, M., Gornicka, A., Koralov, S. B., Szczepanek, K., Morgan, M., Raje, V., . . . Larner, A. C. (2012). Tyk2 and Stat3 Regulate Brown Adipose Tissue Differentiation and Obesity. CELL METABOLISM, 16(6), 814-824. doi:10.1016/j.cmet.2012.11.005

2011

Peterson, T. R., Sengupta, S. S., Harris, T. E., Carmack, A. E., Kang, S. A., Balderas, E., . . . Sabatini, D. M. (2011). mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. CELL, 146(3), 408-420. doi:10.1016/j.cell.2011.06.034

Harris, T. E., & Finck, B. N. (2011). Dual function lipin proteins and glycerolipid metabolism. TRENDS IN ENDOCRINOLOGY AND METABOLISM, 22(6), 226-233. doi:10.1016/j.tem.2011.02.006

Nascimento, N. R. F., Kemp, B. A., Howell, N. L., Gildea, J. J., Santos, C. F., Harris, T. E., & Carey, R. M. (2011). Role of Src Family Kinase in Extracellular Renal Cyclic Guanosine 3â²,5â²-Monophosphate- and Pressure-Induced Natriuresis. HYPERTENSION, 58(1), 107-U211. doi:10.1161/HYPERTENSIONAHA.110.168708

2010

Liu, G. -H., Qu, J., Carmack, A. E., Kim, H. B., Chen, C., Ren, H., . . . Harris, T. E. (2010). Lipin proteins form homo- and hetero-oligomers. BIOCHEMICAL JOURNAL, 432, 65-76. doi:10.1042/BJ20100584

Harris, T. E., Kim, H. B., Kumar, A., Wang, L., Keller, S. R., Finck, B. N., & Lawrence, J. C. J. (2010). Lipin 1 represses NFATc4 transcriptional activity in adipocytes to inhibit secretion of inflammatory factors. FASEB JOURNAL, 24.

Blancquaert, S., Wang, L., Paternot, S., Coulonval, K., Dumont, J. E., Harris, T. E., & Roger, P. P. (2010). cAMP-Dependent Activation of Mammalian Target of Rapamycin (mTOR) in Thyroid Cells. Implication in Mitogenesis and Activation of CDK4. MOLECULAR ENDOCRINOLOGY, 24(7), 1453-1468. doi:10.1210/me.2010-0087

Peterfy, M., Harris, T. E., Fujita, N., & Reue, K. (2010). Insulin-stimulated Interaction with 14-3-3 Promotes Cytoplasmic Localization of Lipin-1 in Adipocytes. JOURNAL OF BIOLOGICAL CHEMISTRY, 285(6), 3857-3864. doi:10.1074/jbc.M109.072488

Kim, H. B., Kumar, A., Wang, L., Liu, G. -H., Keller, S. R., Lawrence, J. C. J., . . . Harris, T. E. (2010). Lipin 1 Represses NFATc4 Transcriptional Activity in Adipocytes To Inhibit Secretion of Inflammatory Factors. MOLECULAR AND CELLULAR BIOLOGY, 30(12), 3126-3139. doi:10.1128/MCB.01671-09

Kumar, A., Lawrence, J. C. J., Jung, D. Y., Ko, H. J., Keller, S. R., Kim, J. K., . . . Harris, T. E. (2010). Fat Cell-Specific Ablation of Rictor in Mice Impairs Insulin-Regulated Fat Cell and Whole-Body Glucose and Lipid Metabolism. DIABETES, 59(6), 1397-1406. doi:10.2337/db09-1061