Lian-Wang Guo


Primary Appointment

Tenured Professor, Surgery


  • PhD, Plant Physiology, Chinese Academy of Sciences

Research Disciplines

Biophysics, Molecular Biology

Research Interests

Pathophysiological mechanisms and impact of cell state transitions

Research Description

Cell plasticity can be physiologic or pathological. Epigenetic effectors transmit environmental cues into chromatin remodeling and cell state transitions. We aspire to understand how their interplay shapes specific cell states. We also do translation research by targeting pathological cell plasticity to treat vascular disease or retinal degeneration.

Selected Publications


Xie, X., Guo, L. -W., & Kent, C. K. (2022). miR548ai antagonism attenuates exosome-induced endothelial cell dysfunction (Oct, 10.1038/s41420-022-01178-z, 2021). CELL DEATH DISCOVERY, 8(1). doi:10.1038/s41420-022-01178-z

Li, J., Felix-Soriano, E., Wright, K. R., Shen, H., Baer, L. A., Stanford, K. I., & Guo, L. -W. (2022). Differential Responses to Sigma-1 or Sigma-2 Receptor Ablation in Adiposity, Fat Oxidation, and Sexual Dimorphism. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23(18). doi:10.3390/ijms231810846

Li, J., Shen, H., Owens, G. K., & Guo, L. -W. (2022). SREBP1 regulates Lgals3 activation in response to cholesterol loading. MOLECULAR THERAPY-NUCLEIC ACIDS, 28, 892-909. doi:10.1016/j.omtn.2022.05.028

Zhang, M., Urabe, G., Ozer, H. G., Xie, X., Webb, A., Shirasu, T., . . . Guo, L. -W. (2022). Angioplasty induces epigenomic remodeling in injured arteries. . Life science alliance, 5(5), e202101114. doi:10.26508/lsa.202101114


Kumar, S., Javed, R., Mudd, M., Pallikkuth, S., Lidke, K. A., Jain, A., . . . Deretic, V. (2021). Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes. CELL, 184(24), 5950-+. doi:10.1016/j.cell.2021.10.017

Xie, X., Guo, L. -W., & Kent, K. C. (2021). miR548ai antagonism attenuates exosome-induced endothelial cell dysfunction. CELL DEATH DISCOVERY, 7(1). doi:10.1038/s41420-021-00720-9

Zhao, Y., Shirasu, T., Yodsanit, N., Kent, E., Ye, M., Wang, Y., . . . Wang, B. (2021). Biomimetic, ROS-detonable nanoclusters-A multimodal nanoplatform for anti-restenotic therapy. JOURNAL OF CONTROLLED RELEASE, 338, 295-306. doi:10.1016/j.jconrel.2021.08.025

Xie, X., Shirasu, T., Guo, L. -W., & Kent, K. C. (2021). Smad2 inhibition of MET transcription potentiates human vascular smooth muscle cell apoptosis. ATHEROSCLEROSIS PLUS, 44, 31-42. doi:10.1016/j.athplu.2021.08.005

Shen, H., Li, J., Heisler-Taylor, T., Makin, R., Yang, H., Mavlyutov, T. A., . . . Guo, L. -W. (2021). TMEM97 ablation aggravates oxidant-induced retinal degeneration. CELLULAR SIGNALLING, 86. doi:10.1016/j.cellsig.2021.110078

Schmitt, H. M., Fehrman, R. L., Maes, M. E., Yang, H., Guo, L. -W., Schlamp, C. L., . . . Nickells, R. W. (2021). Increased Susceptibility and Intrinsic Apoptotic Signaling in Neurons by Induced HDAC3 Expression. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 62(10). doi:10.1167/iovs.62.10.14

Shirasu, T., Yodsanit, N., Xie, X., Zhao, Y., Wang, Y., Xie, R., . . . Kent, K. C. (2021). An adventitial painting modality of local drug delivery to abate intimal hyperplasia. BIOMATERIALS, 275. doi:10.1016/j.biomaterials.2021.120968

Wang, Q., Ozer, H. G., Wang, B., Zhang, M., Urabe, G., Huang, Y., . . . Guo, L. -W. (2021). A hierarchical and collaborative BRD4/CEBPD partnership governs vascular smooth muscle cell inflammation. MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT, 21, 54-66. doi:10.1016/j.omtm.2021.02.021

Zhao, Y., Xie, R., Yodsanit, N., Ye, M., Wang, Y., Wang, B., . . . Gong, S. (2021). Hydrogen peroxide-responsive platelet membrane-coated nanoparticles for thrombus therapy. BIOMATERIALS SCIENCE, 9(7), 2696-2708. doi:10.1039/d0bm02125c

Li, J., Urabe, G., Huang, Y., Zhang, M., Wang, B., Marcho, L., . . . Guo, L. -W. (2021). A Role for Polo-Like Kinase 4 in Vascular Fibroblast Cell-Type Transition. JACC-BASIC TO TRANSLATIONAL SCIENCE, 6(3), 257-283. doi:10.1016/j.jacbts.2020.12.015

Klionsky, D. J., Abdel-Aziz, A. K., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., . . . Tong, C. -K. (2021). Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition). AUTOPHAGY, 17(1), 1-382. doi:10.1080/15548627.2020.1797280

Shen, H., Li, J., Xie, X., Yang, H., Zhang, M., Wang, B., . . . Guo, L. -W. (2021). BRD2 regulation of sigma-2 receptor upon cholesterol deprivation. LIFE SCIENCE ALLIANCE, 4(1). doi:10.26508/lsa.201900540

Yodsanit, N., Wang, B., Zhao, Y., Guo, L. -W., Kent, K. C., & Gong, S. (2021). Recent progress on nanoparticles for targeted aneurysm treatment and imaging. BIOMATERIALS, 265. doi:10.1016/j.biomaterials.2020.120406

Wang, B., Zhang, M., Urabe, G., Shirasu, T., Guo, L. -W., & Kent, K. C. (2021). PERK Inhibition Promotes Post-angioplasty Re-endothelialization via Modulating SMC Phenotype Changes.. The Journal of surgical research, 257, 294-305. doi:10.1016/j.jss.2020.05.070


Yang, H., Shen, H., Li, J., Stanford, K. I., & Guo, L. -W. (2020). Sigma-1 receptor ablation impedes adipocyte-like differentiation of mouse embryonic fibroblasts. CELLULAR SIGNALLING, 75. doi:10.1016/j.cellsig.2020.109732

Huang, Y., Urabe, G., Zhang, M., Li, J., Ozer, H. G., Wang, B., . . . Guo, L. -W. (2020). Nullifying epigenetic writer DOT1L attenuates neointimal hyperplasia. ATHEROSCLEROSIS, 308, 22-31. doi:10.1016/j.atherosclerosis.2020.06.002

Borck, P. C., Guo, L. -W., & Plutzky, J. (2020). BET Epigenetic Reader Proteins in Cardiovascular Transcriptional Programs. CIRCULATION RESEARCH, 126(9), 1190-1208. doi:10.1161/CIRCRESAHA.120.315929

Xie, X., Urabe, G., Marcho, L., Williams, C., Guo, L. -W., & Kent, K. C. (2020). Smad3 Regulates Neuropilin 2 Transcription by Binding to its 5 ' Untranslated Region. JOURNAL OF THE AMERICAN HEART ASSOCIATION, 9(8). doi:10.1161/JAHA.119.015487

Wang, B., Zhang, M., Urabe, G., Huang, Y., Chen, G., Wheeler, D., . . . Kent, K. C. (2020). PERK Inhibition Mitigates Restenosis and Thrombosis A Potential Low-Thrombogenic Antirestenotic Paradigm. JACC-BASIC TO TRANSLATIONAL SCIENCE, 5(3), 245-263. doi:10.1016/j.jacbts.2019.12.005


Xie, X., Urabe, G., Marcho, L., Stratton, M., Guo, L. -W., & Kent, C. K. (2019). ALDH1A3 Regulations of Matricellular Proteins Promote Vascular Smooth Muscle Cell Proliferation. ISCIENCE, 19, 872-+. doi:10.1016/j.isci.2019.08.044

Yang, H., Shen, H., Li, J., & Guo, L. -W. (2019). SIGMAR1/Sigma-1 receptor ablation impairs autophagosome clearance. AUTOPHAGY, 15(9), 1539-1557. doi:10.1080/15548627.2019.1586248

Zhang, M., Wang, B., Urabe, G., Huang, Y., Plutzky, J., Kent, K. C., & Guo, L. -W. (2019). The BD2 domain of BRD4 is a determinant in EndoMT and vein graft neointima formation. CELLULAR SIGNALLING, 61, 20-29. doi:10.1016/j.cellsig.2019.05.005

Zhang, J., McIntosh, B. E., Wang, B., Brown, M. E., Probasco, M. D., Webster, S., . . . Thomson, J. A. (2019). A Human Pluripotent Stem Cell-Based Screen for Smooth Muscle Cell Differentiation and Maturation Identifies Inhibitors of Intimal Hyperplasia. STEM CELL REPORTS, 12(6), 1269-1281. doi:10.1016/j.stemcr.2019.04.013

Shi, Y., Johnson, J., Wang, B., Chen, B., Fisher, G. L., Urabe, G., . . . Li, L. (2019). Mass Spectrometric Imaging Reveals Temporal and Spatial Dynamics of Bioactive Lipids in Arteries Undergoing Restenosis. JOURNAL OF PROTEOME RESEARCH, 18(4), 1669-1678. doi:10.1021/acs.jproteome.8b00941


Zhang, M., Urabe, G., Little, C., Wang, B., Kent, A. M., Huang, Y., . . . Guo, L. -W. (2018). HDAC6 Regulates the MRTF-A/SRF Axis and Vascular Smooth Muscle Cell Plasticity. JACC-BASIC TO TRANSLATIONAL SCIENCE, 3(6), 782-795. doi:10.1016/j.jacbts.2018.08.010

Wang, B., Chen, G., Urabe, G., Xie, R., Wang, Y., Shi, X., . . . Kent, K. C. (2018). A paradigm of endothelium-protective and stent-free anti-restenotic therapy using biomimetic nanoclusters. BIOMATERIALS, 178, 293-301. doi:10.1016/j.biomaterials.2018.06.025

Mavylutov, T., Chen, X., Guo, L., & Yang, J. (2018). APEX2-tagging of Sigma 1-receptor indicates subcellular protein topology with cytosolic N-terminus and ER luminal C-terminus. PROTEIN & CELL, 9(8), 733-737. doi:10.1007/s13238-017-0468-5

Pan, X., Wang, B., Yuan, T., Zhang, M., Kent, K. C., & Guo, L. -W. (2018). Analysis of Combined Transcriptomes Identifies Gene Modules that Differentially Respond to Pathogenic Stimulation of Vascular Smooth Muscle and Endothelial Cells. SCIENTIFIC REPORTS, 8. doi:10.1038/s41598-017-18675-2

Zent, J., & Guo, L. -W. (2018). Signaling Mechanisms of Myofibroblastic Activation: Outside-in and Inside-Out. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 49(3), 848-868. doi:10.1159/000493217


Yang, H., Fu, Y., Liu, X., Shahi, P. K., Mavlyutov, T. A., Li, J., . . . Guo, L. -W. (2017). Role of the sigma-1 receptor chaperone in rod and cone photoreceptor degenerations in a mouse model of retinitis pigmentosa. MOLECULAR NEURODEGENERATION, 12. doi:10.1186/s13024-017-0202-z

Yu, Q., Wang, B., Chen, Z., Urabe, G., Glover, M. S., Shi, X., . . . Li, L. (2017). Electron-Transfer/Higher-Energy Collision Dissociation (EThcD)-Enabled Intact Glycopeptide/Glycoproteome Characterization. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 28(9), 1751-1764. doi:10.1007/s13361-017-1701-4

Liu, X., Fu, Y., Yang, H., Mavlyutov, T., Li, J., McCurdy, C. R., . . . Pattnaik, B. R. (2017). Potential independent action of sigma receptor ligands through inhibition of the Kv2.1 channel. ONCOTARGET, 8(35), 59345-59358. doi:10.18632/oncotarget.19581

Mavlyutov, T. A., Yang, H., Epstein, M. L., Ruoho, A. E., Yang, J., & Guo, L. -W. (2017). APEX2-enhanced electron microscopy distinguishes sigma-1 receptor localization in the nucleoplasmic reticulum. ONCOTARGET, 8(31), 51317-51330. doi:10.18632/oncotarget.17906

Chen, G., Shi, X., Wang, B., Xie, R., Guo, L. -W., Gong, S., & Kent, K. C. (2017). Unimolecular Micelle-Based Hybrid System for Perivascular Drug Delivery Produces Long-Term Efficacy for Neointima Attenuation in Rats. BIOMACROMOLECULES, 18(7), 2205-2213. doi:10.1021/acs.biomac.7b00617

Kumar, A., D'Souza, S. S., Moskvin, O. V., Toh, H., Wang, B., Zhang, J., . . . Slukvin, I. I. (2017). Specification and Diversification of Pericytes and Smooth Muscle Cells from Mesenchymoangioblasts. CELL REPORTS, 19(9), 1902-1916. doi:10.1016/j.celrep.2017.05.019

Li, J., Zhao, L., Urabe, G., Fu, Y., & Guo, L. -W. (2017). Epigenetic intervention with a BET inhibitor ameliorates acute retinal ganglion cell death in mice. MOLECULAR VISION, 23, 149-159. Retrieved from

Zhao, L., Chen, G., Li, J., Fu, Y., Mavlyutov, T. A., Yao, A., . . . Guo, L. -W. (2017). An intraocular drug delivery system using targeted nanocarriers attenuates retinal ganglion cell degeneration. JOURNAL OF CONTROLLED RELEASE, 247, 153-166. doi:10.1016/j.jconrel.2016.12.038

Zhu, Y., Takayama, T., Wang, B., Kent, A., Zhang, M., Binder, B. Y. K., . . . Guo, L. -W. (2017). Restenosis Inhibition and Re-differentiation of TGF beta/Smad3-activated Smooth Muscle Cells by Resveratrol. SCIENTIFIC REPORTS, 7. doi:10.1038/srep41916

Zhao, L., Li, J., Fu, Y., Zhang, M., Wang, B., Ouellette, J., . . . Guo, L. -W. (2017). Photoreceptor protection via blockade of BET epigenetic readers in a murine model of inherited retinal degeneration. JOURNAL OF NEUROINFLAMMATION, 14. doi:10.1186/s12974-016-0775-4

Mavlyutov, T. A., & Guo, L. -W. (2017). Peeking into Sigma-1 Receptor Functions Through the Retina. SIGMA RECEPTORS: THEIR ROLE IN DISEASE AND AS THERAPEUTIC TARGETS, 964, 285-297. doi:10.1007/978-3-319-50174-1_19


Shi, X., Guo, L. -W., Seedial, S., Takayama, T., Wang, B., Zhang, M., . . . Kent, K. C. (2016). Local CXCR4 Upregulation in the Injured Arterial Wall Contributes to Intimal Hyperplasia. STEM CELLS, 34(11), 2744-2757. doi:10.1002/stem.2442

Chaudhary, M. A., Guo, L. -W., Shi, X., Chen, G., Gong, S., Liu, B., & Kent, K. C. (2016). Periadventitial drug delivery for the prevention of intimal hyperplasia following open surgery. JOURNAL OF CONTROLLED RELEASE, 233, 174-180. doi:10.1016/j.jconrel.2016.05.002

DiRenzo, D. M., Chaudhary, M. A., Shi, X., Franco, S. R., Zent, J., Wang, K., . . . Kent, K. C. (2016). A crosstalk between TGF-beta/Smad3 and Wnt/beta-catenin pathways promotes vascular smooth muscle cell proliferation. CELLULAR SIGNALLING, 28(5), 498-505. doi:10.1016/j.cellsig.2016.02.011

Abate, C., Riganti, C., Pati, M. L., Ghigo, D., Berardi, F., Mavlyutov, T., . . . Ruoho, A. (2016). Development of sigma-1 (sigma(1)) receptor fluorescent ligands as versatile tools to study sigma(1) receptors. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 108, 577-585. doi:10.1016/j.ejmech.2015.12.014


Chu, U. B., Mavlyutov, T. A., Chu, M. -L., Yang, H., Schulman, A., Mesangeau, C., . . . Ruoho, A. E. (2015). The Sigma-2 Receptor and Progesterone Receptor Membrane Component 1 are Different Binding Sites Derived From Independent Genes. EBIOMEDICINE, 2(11), 1806-1813. doi:10.1016/j.ebiom.2015.10.017

Wang, B., Zhang, M., Takayama, T., Shi, X., Roenneburg, D. A., Kent, K. C., & Guo, L. -W. (2015). BET Bromodomain Blockade Mitigates Intimal Hyperplasia in Rat Carotid Arteries. EBIOMEDICINE, 2(11), 1650-1661. doi:10.1016/j.ebiom.2015.09.045

Mavlyutov, T. A., Epstein, M., & Guo, L. -W. (2015). Subcellular Localization of the Sigma-1 Receptor in Retinal Neurons - an Electron Microscopy Study. SCIENTIFIC REPORTS, 5. doi:10.1038/srep10689

Tsai, T. -L., Wang, B., Squire, M. W., Guo, L. -W., & Li, W. -J. (2015). Endothelial cells direct human mesenchymal stem cells for osteo- and chondro-lineage differentiation through endothelin-1 and AKT signaling. STEM CELL RESEARCH & THERAPY, 6. doi:10.1186/s13287-015-0065-6

Takayama, T., Shi, X., Wang, B., Franco, S., Zhou, Y., DiRenzo, D., . . . Guo, L. -W. (2015). A Murine Model of Arterial Restenosis: Technical Aspects of Femoral Wire Injury. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (97). doi:10.3791/52561

Mavlyutov, T. A., Guo, L. -W., Epstein, M. L., & Ruoho, A. E. (2015). Role of the Sigma-1 receptor in Amyotrophic Lateral Sclerosis (ALS). JOURNAL OF PHARMACOLOGICAL SCIENCES, 127(1), 10-16. doi:10.1016/j.jphs.2014.12.013


Yu, X., Takayama, T., Goel, S. A., Shi, X., Zhou, Y., Kent, K. C., . . . Guo, L. -W. (2014). A rapamycin-releasing perivascular polymeric sheath produces highly effective inhibition of intimal hyperplasia. JOURNAL OF CONTROLLED RELEASE, 191, 47-53. doi:10.1016/j.jconrel.2014.05.017

Guo, L. -W., Wang, B., Goel, S. A., Little, C., Takayama, T., Shi, X. D., . . . Kent, K. C. (2014). Halofuginone Stimulates Adaptive Remodeling and Preserves Re-Endothelialization in Balloon-Injured Rat Carotid Arteries. CIRCULATION-CARDIOVASCULAR INTERVENTIONS, 7(4), 594-+. doi:10.1161/CIRCINTERVENTIONS.113.001181

Shi, X., Guo, L. -W., Seedial, S. M., Si, Y., Wang, B., Takayama, T., . . . Kent, K. C. (2014). TGF-beta/Smad3 inhibit vascular smooth muscle cell apoptosis through an autocrine signaling mechanism involving VEGF-A. CELL DEATH & DISEASE, 5. doi:10.1038/cddis.2014.282

Shi, X., DiRenzo, D., Guo, L. -W., Franco, S. R., Wang, B., Seedial, S., & Kent, K. C. (2014). TGF-beta/Smad3 Stimulates Stem Cell/Developmental Gene Expression and Vascular Smooth Muscle Cell De-Differentiation. PLOS ONE, 9(4). doi:10.1371/journal.pone.0093995

Goel, S. A., Guo, L. -W., Wang, B., Guo, S., Roenneburg, D., Ananiev, G. E., . . . Kent, K. C. (2014). High-Throughput Screening Identifies Idarubicin as a Preferential Inhibitor of Smooth Muscle versus Endothelial Cell Proliferation. PLOS ONE, 9(2). doi:10.1371/journal.pone.0089349

Shi, X., Chen, G., Guo, L. -W., Si, Y., Zhu, M., Pilla, S., . . . Kent, K. C. (2014). Periadventitial Application of Rapamycin-Loaded Nanoparticles Produces Sustained Inhibition of Vascular Restenosis. PLOS ONE, 9(2). doi:10.1371/journal.pone.0089227


Goel, S. A., Guo, L. -W., Shi, X. -D., Kundi, R., Sovinski, G., Seedial, S., . . . Kent, K. C. (2013). Preferential secretion of collagen type 3 versus type 1 from adventitial fibroblasts stimulated by TGF-beta/Smad3-treated medial smooth muscle cells. CELLULAR SIGNALLING, 25(4), 955-960. doi:10.1016/j.cellsig.2012.12.021


Goel, S. A., Guo, L. -W., Liu, B., & Kent, K. C. (2012). Mechanisms of post-intervention arterial remodelling. CARDIOVASCULAR RESEARCH, 96(3), 363-371. doi:10.1093/cvr/cvs276

Guo, L. -W., Hajipour, A. R., Karaoglu, K., Mavlyutov, T. A., & Ruoho, A. E. (2012). Development of Benzophenone-Alkyne Bifunctional Sigma Receptor Ligands. CHEMBIOCHEM, 13(15), 2277-2289. doi:10.1002/cbic.201200427


Hajipour, A. R., Guo, L. -W., Pal, A., Mavlyutov, T., & Ruoho, A. E. (2011). Electron-donating para-methoxy converts a benzamide-isoquinoline derivative into a highly Sigma-2 receptor selective ligand. BIOORGANIC & MEDICINAL CHEMISTRY, 19(24), 7435-7440. doi:10.1016/j.bmc.2011.10.046

Guo, L. -W., & Ruoho, A. E. (2011). N-terminal Half of the cGMP Phosphodiesterase gamma-Subunit Contributes to Stabilization of the GTPase-accelerating Protein Complex. JOURNAL OF BIOLOGICAL CHEMISTRY, 286(17), 15260-15267. doi:10.1074/jbc.M110.210567

Mavlyutov, T. A., Nickells, R. W., & Guo, L. -W. (2011). Accelerated retinal ganglion cell death in mice deficient in the Sigma-1 receptor. MOLECULAR VISION, 17(116), 1034-1043. Retrieved from


Chu, U. B., Song, J., Mavlyutov, T. A., & Guo, L. -W. (2010). In vitro interaction of tubulin with the photoreceptor cGMP phosphodiesterase gamma-subunit. NEUROSCIENCE LETTERS, 482(3), 225-229. doi:10.1016/j.neulet.2010.07.044

Guo, L. -W., Hajipour, A. R., & Ruoho, A. E. (2010). Complementary Interactions of the Rod PDE6 Inhibitory Subunit with the Catalytic Subunits and Transducin. JOURNAL OF BIOLOGICAL CHEMISTRY, 285(20), 15209-15219. doi:10.1074/jbc.M109.086116