Urszula Derewenda

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Primary Appointment

Assistant Professor of Research, Molecular Physiology and Biological Physics

Research Interests

Our primary interest is in the mechanisms by which protein molecules accomplish their diverse biological tasks.

Research Description

Our primary interest is in the mechanisms by which protein molecules accomplish their diverse biological tasks. Crystallography and molecular biology are the primary tools with which we probe into the structure-function relationships in complex proteins. There are two major themes in our work: structural biology of cytoskeletal regulation and the mechanism of hydrolytic enzymes, such as esterases and thioesterases.

Personal Statement

Our primary interest is in the mechanisms by which protein molecules accomplish their diverse biological tasks. Crystallography and molecular biology are the primary tools with which we probe into the structure-function relationships in complex proteins. There are two major themes in our work: structural biology of cytoskeletal regulation and the mechanism of hydrolytic enzymes, such as esterases and thioesterases.

Selected Publications

Zheng M, Cierpicki T, Burdette AJ, Utepbergenov D, Janczyk PÅ, Derewenda U, Stukenberg PT, Caldwell KA, Derewenda ZS, Structural features and chaperone activity of the NudC protein family., 2011; Journal of molecular biology. 409(5) 722-41. PMID: 21530541 | PMCID: PMC3159028

ZyÅkiewicz E, KijaÅska M, Choi WC, Derewenda U, Derewenda ZS, Stukenberg PT, The N-terminal coiled-coil of Ndel1 is a regulated scaffold that recruits LIS1 to dynein., 2011; The Journal of cell biology. 192(3) 433-45. PMID: 21282465 | PMCID: PMC3101096

Cierpicki T, Bielnicki J, Zheng M, Gruszczyk J, Kasterka M, Petoukhov M, Zhang A, Fernandez EJ, Svergun DI, Derewenda U, Bushweller JH, Derewenda ZS, The solution structure and dynamics of the DH-PH module of PDZRhoGEF in isolation and in complex with nucleotide-free RhoA., 2009; Protein science : a publication of the Protein Society. 18(10) 2067-79. PMID: 19670212 | PMCID: PMC2786971

Derewenda U, Boczek T, Gorres KL, Yu M, Hung LW, Cooper D, Joachimiak A, Raines RT, Derewenda ZS, Structure and function of Bacillus subtilis YphP, a prokaryotic disulfide isomerase with a CXC catalytic motif ., 2009; Biochemistry. 48(36) 8664-71. PMID: 19653655 | PMCID: PMC2739605

Zheng M, Cierpicki T, Momotani K, Artamonov MV, Derewenda U, Bushweller JH, Somlyo AV, Derewenda ZS, On the mechanism of autoinhibition of the RhoA-specific nucleotide exchange factor PDZRhoGEF., 2009; BMC structural biology. 9() 36. PMID: 19460155 | PMCID: PMC2695464

Zheng M, Cooper DR, Grossoehme NE, Yu M, Hung LW, Cieslik M, Derewenda U, Lesley SA, Wilson IA, Giedroc DP, Derewenda ZS, Structure of Thermotoga maritima TM0439: implications for the mechanism of bacterial GntR transcription regulators with Zn2+-binding FCD domains., 2009; Acta crystallographica. Section D, Biological crystallography. 65(0) 356-65. PMID: 19307717 | PMCID: PMC2659884

Guergnon J, Derewenda U, Edelson JR, Brautigan DL, Mapping of protein phosphatase-6 association with its SAPS domain regulatory subunit using a model of helical repeats., 2009; BMC biochemistry. 10() 24. PMID: 19835610 | PMCID: PMC2765987

Smietana K, Kasztura M, Paduch M, Derewenda U, Derewenda ZS, Otlewski J, Degenerate specificity of PDZ domains from RhoA-specific nucleotide exchange factors PDZRhoGEF and LARG., 2008; Acta biochimica Polonica. 55(2) 269-80. PMID: 18542831

Derewenda U, Tarricone C, Choi WC, Cooper DR, Lukasik S, Perrina F, Tripathy A, Kim MH, Cafiso DS, Musacchio A, Derewenda ZS, The structure of the coiled-coil domain of Ndel1 and the basis of its interaction with Lis1, the causal protein of Miller-Dieker lissencephaly., 2007; Structure (London, England : 1993). 15(11) 1467-81. PMID: 17997972

Oleksy A, OpaliÅski Å, Derewenda U, Derewenda ZS, Otlewski J, The molecular basis of RhoA specificity in the guanine nucleotide exchange factor PDZ-RhoGEF., 2006; The Journal of biological chemistry. 281(43) 32891-7. PMID: 16954208

Cierpicki T, Kim MH, Cooper DR, Derewenda U, Bushweller JH, Derewenda ZS, The DC-module of doublecortin: dynamics, domain boundaries, and functional implications., 2006; Proteins. 64(4) 874-82. PMID: 16835924

Grembecka J, Cierpicki T, Devedjiev Y, Derewenda U, Kang BS, Bushweller JH, Derewenda ZS, The binding of the PDZ tandem of syntenin to target proteins., 2006; Biochemistry. 45(11) 3674-83. PMID: 16533050

Cierpicki T, Kim MH, Cooper DR, Derewenda U, Bushweller JH, Derewenda ZS, The DC-module of doublecortin: dynamics, domain boundaries, and functional implications., 2006; Proteins. 64(4) 874-82. PMID:

Derewenda U, Oleksy A, Stevenson AS, Korczynska J, Dauter Z, Somlyo AP, Otlewski J, Somlyo AV, Derewenda ZS, The crystal structure of RhoA in complex with the DH/PH fragment of PDZRhoGEF, an activator of the Ca(2+) sensitization pathway in smooth muscle., 2004; Structure (London, England : 1993). 12(11) 1955-65. PMID: 15530360

Janda I, Devedjiev Y, Derewenda U, Dauter Z, Bielnicki J, Cooper DR, Graf PC, Joachimiak A, Jakob U, Derewenda ZS, The crystal structure of the reduced, Zn2+-bound form of the B. subtilis Hsp33 chaperone and its implications for the activation mechanism., 2004; Structure (London, England : 1993). 12(10) 1901-7. PMID: 15458638 | PMCID: PMC3691021