Joshua D Wythe
- Email: jwythe@virginia.edu
- Website: https://www.wythelab.com/
Primary Appointment
Associate Professor, Cell and Developmental Biology
Education
- BA, History, Miami University
- BS, Botany, Miami University
- PhD, Oncological Sciences / Cardiovascular Development, University of Utah School of Medicine
- Postdoctoral Fellowship, Cardiovascular Development and Disease, University of California, San Francisco
Research Disciplines
Cardiovascular Biology, Cell and Developmental Biology, Genetics, Neuroscience, Translational Science
Research Interests
Cardiovascular Development and Cerebrovascular Pathologies
Research Description
underlying congenital birth defects, as well as for developing therapeutics to combat cardiovascular disease: the leading cause of mortality and morbidity in the world.
The main focus of our research is to understand the molecular, genetic, and cellular mechanisms underpinning the formation, function, and maintenance of blood vessels in the developing vertebrate embryo, while simultaneously understanding how these factors are dysregulated in pathological settings in the adult, with a focus on diseases that impact the brain, such as stroke, vascular dementia, and brain cancer. We combine both zebrafish and mouse genetic models together with bioinformatics, functional genomics, and 3D imaging to investigate blood vessel development and pathogenesis. We are currently pursuing three main projects in our laboratory, among others:
1) Defining the transcriptional basis of endothelial plasticity and function.
Vessels of different organs have unique properties, such as the impermeable nature of the brain endothelium (e.g. the blood brain barrier or BBB) versus the porous, fenestrated endothelium of the liver. The basis for this heterogeneity is unknown. Through bulk and single cell transcriptional and epigenetic (ATAC-seq) profiling and informatic analyses we have identified a set of core transcription factors unique the vessels of each major organ. We are now focusing on the transcription factors that govern BBB acquisition, and determining if these same factors can reprogram the functional characteristics of vessels in other organs to make them adopt different functional properties and characteristics.
2) The role of RAS/MEK/ERK signaling in brain arteriovenous malformations.
We recently found that endothelial-specific gain of function mutations in KRAS are present in brain arteriovenous malformations: shunts or abnormal connections between arteries and veins that lack an intervening capillary network. These shunts are fragile and prone to rupture, which leads to intracerebral hemorrhage and possibly death. We are now asking if targeting this pathway can block or reverse these lesions, while also pursuing the mechanisms of KRAS-induced bAVMs at the cellular and molecular level.
3) Identifying novel therapeutic vulnerabilities in glioblastoma.
Excessive endothelial cell proliferation and sprouting are defining features of the deadly adult brain cancer, glioblastoma multiforme (GBM). Our work has demonstrated that blood vessels in GBM display structural and functional heterogeneity. Currently, we determining if developmental angiogenic regulators weâve identified also regulate pathogenic angiogenesis in this setting, and whether these factors can be targeted to inhibit tumor vascularization, and thus disease progression.
Other projects include modelling vascular dementia in mice, creating novel animal models of lymphatic vessel malformations in mice and zebrafish, and creating novel animal models of inherited and sporadic diseases that feature cardiovascular defects.
At the heart of these projects is a concerted effort to identity the transcriptional regulators and molecular networks that endow endothelium with their unique functional characteristics (such as impressive barrier function of the BBB in the brain). To achieve this goal requires a detailed mechanistic understanding of how endothelial cell identity is specified and maintained and the long-term objective of our research is to gain a deeper understanding of these mechanisms to facilitate the repair or replacement of damaged or dysfunctional vessels, or alternatively prevent exuberant vascularization in disease settings, such as glioma.
Personal Statement
Cardiovascular Development
CNS Vascular Development and Disease
Angiogenesis in Glioblastoma (brain tumors)
Brain Arteriovenous Malformations
Vascular Dementia
3D Imaging and Data Analysis
Transcription and Epigenetics
Murine and Zebrafish models of human disease
Selected Publications
2023
Long, H., Steimle, J., Canozo, F. J. G., Kim, J. H., Li, X., Morikawa, Y., . . . Martin, J. F. (2024). Endothelial cells adopt a pro-reparative immune responsive signature during cardiac injury. LIFE SCIENCE ALLIANCE, 7(2). doi:10.26508/lsa.202201870
Kim, G. S., Harmon, E., Gutierrez, M., Stephenson, J., Chauhan, A., Banerjee, A., . . . Marrelli, S. (2023). Single-cell analysis identifies Ifi27l2a as a novel gene regulator of microglial inflammation in the context of aging and stroke.. Res Sq. doi:10.21203/rs.3.rs-2557290/v1
Ricciardelli, A. R., Robledo, A., Fish, J. E., Kan, P. T., Harris, T. H., & Wythe, J. D. (2023). The Role and Therapeutic Implications of Inflammation in the Pathogenesis of Brain Arteriovenous Malformations. BIOMEDICINES, 11(11). doi:10.3390/biomedicines11112876
2022
Hsu, C. -W., Cerda, J., Kirk, J. M., Turner, W. D., Rasmussen, T. L., Suarez, C. P. F., . . . Wythe, J. D. (2022). EZ Clear for simple, rapid, and robust mouse whole organ clearing. ELIFE, 11. doi:10.7554/eLife.77419
Verma, S. K., Deshmukh, V., Thatcher, K., Belanger, K. K., Rhyner, A. M., Meng, S., . . . Kuyumcu-Martinez, M. N. (2022). RBFOX2 is required for establishing RNA regulatory networks essential for heart development. NUCLEIC ACIDS RESEARCH, 50(4), 2270-2286. doi:10.1093/nar/gkac055
Mayerich, D., & Wythe, J. D. (2022). Computational insights on coronary artery function. NATURE CARDIOVASCULAR RESEARCH, 1(8), 691-693. doi:10.1038/s44161-022-00115-8
Soon, K., Li, M., Wu, R., Zhou, A., Khosraviani, N., Turner, W. D., . . . Nunes, S. S. (2022). A human model of arteriovenous malformation (AVM)-on-a-chip reproduces key disease hallmarks and enables drug testing in perfused human vessel networks. BIOMATERIALS, 288. doi:10.1016/j.biomaterials.2022.121729
Rohde, D., Vandoorne, K., Lee, I. -H., Grune, J., Zhang, S., McAlpine, C. S., . . . Nahrendorf, M. (2022). Bone marrow endothelial dysfunction promotes myeloid cell expansion in cardiovascular disease. NATURE CARDIOVASCULAR RESEARCH, 1(1), 28-+. doi:10.1038/s44161-021-00002-8
Li-Villarreal, N., Wong, R. L. Y., Garcia, M. D., Udan, R. S., Poche, R. A., Rasmussen, T. L., . . . Dickinson, M. E. (2022). FOXO1 represses sprouty 2 and sprouty 4 expression to promote arterial specification and vascular remodeling in the mouse yolk sac. DEVELOPMENT, 149(7). doi:10.1242/dev.200131
2021
Shao, Y., Bajikar, S. S., Tirumala, H. P., Gutierrez, M. C., Wythe, J. D., & Zoghbi, H. Y. (2021). Identification and characterization of conserved noncoding cis-regulatory elements that impact Mecp2 expression and neurological functions. GENES & DEVELOPMENT, 35(7-8), 489-494. doi:10.1101/gad.345397.120
2020
Carlson, J. C., Gutierrez, M. C., Lozzi, B., Huang-Hobbs, E., Turner, W. D., Tepe, B., . . . Deneen, B. (2021). Identification of diverse tumor endothelial cell populations in malignant glioma. NEURO-ONCOLOGY, 23(6), 932-944. doi:10.1093/neuonc/noaa297
Fish, J. E., Flores-Suarez, C. P., Boudreau, E., Herman, A. M., Gutierrez, M. C., Gustafson, D., . . . Wythe, J. D. (2020). Somatic Gain of KRAS Function in the Endothelium Is Sufficient to Cause Vascular Malformations That Require MEK but Not PI3K Signaling. CIRCULATION RESEARCH, 127(6), 727-743. doi:10.1161/CIRCRESAHA.119.316500
Ellis, L. V., Cain, M. P., Hutchison, V., Flodby, P., Crandall, E. D., Borok, Z., . . . Chen, J. (2020). Epithelial Vegfa Specifies a Distinct Endothelial Population in the Mouse Lung. DEVELOPMENTAL CELL, 52(5), 617-+. doi:10.1016/j.devcel.2020.01.009
Hong, S. -H., Herman, A. M., Stephenson, J. M., Wu, T., Bahadur, A. N., Burns, A. R., . . . Wythe, J. D. (2020). Development of barium-based low viscosity contrast agents for micro CT vascular casting: Application to 3D visualization of the adult mouse cerebrovasculature. JOURNAL OF NEUROSCIENCE RESEARCH, 98(2), 312-324. doi:10.1002/jnr.24539
Geng, X., Yanagida, K., Akwii, R. G., Choi, D., Chen, L., Ho, Y., . . . Srinivasan, R. S. (2020). S1PR1 regulates the quiescence of lymphatic vessels by inhibiting laminar shear stress-dependent VEGF-C signaling. JCI INSIGHT, 5(14). doi:10.1172/jci.insight.137652
2019
Gutierrez, A. C., Gutierrez, M. C., Rhyner, A. M., Ruiz, O. E., Eisenhoffer, G. T., & Wythe, J. D. (2019). FishNET: An automated relational database for zebrafish colony management. PLOS BIOLOGY, 17(6). doi:10.1371/journal.pbio.3000343
Hill, M. C., Kadow, Z. A., Li, L., Tran, T. T., Wythe, J. D., & Martin, J. F. (2019). A cellular atlas of Pitx2-dependent cardiac development. DEVELOPMENT, 146(12). doi:10.1242/dev.180398
2018
Xiao, Y., Hill, M. C., Zhang, M., Martin, T. J., Morikawa, Y., Wang, S., . . . Martin, J. F. (2018). Hippo Signaling Plays an Essential Role in Cell State Transitions during Cardiac Fibroblast Development. DEVELOPMENTAL CELL, 45(2), 153-+. doi:10.1016/j.devcel.2018.03.019
Nikolaev, S. I., Vetiska, S., Bonilla, X., Boudreau, E., Jauhiainen, S., Jahromi, B. R., . . . Radovanovic, I. (2018). Somatic Activating KRAS Mutations in Arteriovenous Malformations of the Brain. NEW ENGLAND JOURNAL OF MEDICINE, 378(3), 250-261. doi:10.1056/NEJMoa1709449
Herman, A. M., Rhyner, A. M., Devine, W. P., Marrelli, S. P., Bruneau, B. G., & Wythe, J. D. (2018). A novel reporter allele for monitoring Dll4 expression within the embryonic and adult mouse. BIOLOGY OPEN, 7(3). doi:10.1242/bio.026799
Wang, K., Zhao, S., Liu, B., Zhang, Q., Li, Y., Liu, J., . . . Wu, N. (2018). Perturbations of BMP/TGF-β and VEGF/VEGFR signalling pathways in non-syndromic sporadic brain arteriovenous malformations (BAVM). JOURNAL OF MEDICAL GENETICS, 55(10), 675-684. doi:10.1136/jmedgenet-2017-105224
2017
Fish, J. E., Gutierrez, M. C., Dang, L. T., Khyzha, N., Chen, Z., Veitch, S., . . . Wythe, J. D. (2017). Dynamic regulation of VEGF-inducible genes by an ERK/ERG/p300 transcriptional network. DEVELOPMENT, 144(13), 2428-2444. doi:10.1242/dev.146050
2016
Liu, Q., Zhang, H., Tian, X., He, L., Huang, X., Tan, Z., . . . Zhou, B. (2016). Smooth muscle origin of postnatal 2nd CVP is pre-determined in early embryo. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 471(4), 430-436. doi:10.1016/j.bbrc.2016.02.062
He, L., Liu, Q., Hu, T., Huang, X., Zhang, H., Tian, X., . . . Zhou, B. (2016). Genetic lineage tracing discloses arteriogenesis as the main mechanism for collateral growth in the mouse heart. CARDIOVASCULAR RESEARCH, 109(3), 419-430. doi:10.1093/cvr/cvw005
Wang, J., Xiao, Y., Hsu, C. -W., Martinez-Traverso, I. M., Zhang, M., Bai, Y., . . . Martin, J. F. (2016). Yap and Taz play a crucial role in neural crest-derived craniofacial development. DEVELOPMENT, 143(3), 504-515. doi:10.1242/dev.126920
2015
Fish, J. E., & Wythe, J. D. (2015). The Molecular Regulation of Arteriovenous Specification and Maintenance. DEVELOPMENTAL DYNAMICS, 244(3), 391-409. doi:10.1002/dvdy.24252
Liu, Q., Hu, T., He, L., Huang, X., Tian, X., Zhang, H., . . . Zhou, B. (2015). Genetic targeting of sprouting angiogenesis using Apln-CreER. Nature Communications, 6. doi:10.1038/ncomms7020
Lizama, C. O., Hawkins, J. S., Schmitt, C. E., Bos, F. L., Zape, J. P., Cautivo, K. M., . . . Zovein, A. C. (2015). Repression of arterial genes in hemogenic endothelium is sufficient for haematopoietic fate acquisition. NATURE COMMUNICATIONS, 6. doi:10.1038/ncomms8739
2014
Devine, W. P., Wythe, J. D., George, M., Koshiba-Takeuchi, K., & Bruneau, B. G. (2014). Early Patterning and Specification of Cardiac Progenitors in Gastrulating Mesoderm. ELIFE, 3. doi:10.7554/eLife.03848
He, L., Tian, X., Zhang, H., Wythe, J. D., & Zhou, B. (2014). Fabp4-CreER lineage tracing reveals two distinctive coronary vascular populations. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 18(11), 2152-2156. doi:10.1111/jcmm.12415
2013
Wythe, J. D., Dang, L. T. H., Devine, W. P., Boudreau, E., Artap, S. T., He, D., . . . Fish, J. E. (2013). ETS Factors Regulate Vegf-Dependent Arterial Specification. DEVELOPMENTAL CELL, 26(1), 45-58. doi:10.1016/j.devcel.2013.06.007
2011
Fish, J. E., Wythe, J. D., Xiao, T., Bruneau, B. G., Stainier, D. Y. R., Srivastava, D., & Woo, S. (2011). A Slit/miR-218/Robo regulatory loop is required during heart tube formation in zebrafish. DEVELOPMENT, 138(7), 1409-1419. doi:10.1242/dev.060046
Qian, L., Wythe, J. D., Liu, J., Cartry, J., Vogler, G., Mohapatra, B., . . . Bodmer, R. (2011). Tinman/Nkx2-5 acts via miR-1 and upstream of Cdc42 to regulate heart function across species. JOURNAL OF CELL BIOLOGY, 193(7), 1181-1196. doi:10.1083/jcb.201006114
Wythe, J. D., Jurynec, M. J., Urness, L. D., Jones, C. A., Sabeh, M. K., Werdich, A. A., . . . Li, D. Y. (2011). Hadp1, a newly identified pleckstrin homology domain protein, is required for cardiac contractility in zebrafish. DISEASE MODELS & MECHANISMS, 4(5), 607-621. doi:10.1242/dmm.002204