Wolf Lab

Robert M. Berne Cardiovascular Research Center
Division of Cardiovascular Medicine
University of Virginia Health System

Publications

Cover Images

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Circulation Research Cover 2011

Science Signaling Cover 2015

JCDD Cover 2022

Media

While more research is needed, harmine, which is found in the plant Syrian rue, may be effective in treating patients in recovery following a heart attack. The full article can be found at https://news.virginia.edu, click here to view it.

List of Publications

    Original manuscripts in peer-reviewed journals

  1. Chakraborty, A., Peterson, N.G., King, J.S., Gross, R., Plá, M.M., Thennavan, A., Zhou, K.C., DeLuca, S., Bursac, N., Bowles, D.E., Wolf, M.J. and Fox, D.T. Conserved Chamber-Specific Polyploidy Maintains Heart Function in Drosophila. Development (2023).

  2. Nakhaei-Rad, S., Bazgir, F., Haghighi, F., Bucholzer, M., Dahlmann, J., Schänzer, A., Hahn, A., Kötter, S., Schanze, D., Anand, R., Funk, F., Borchardt, A., Kronenbitter, A.V., Scheller, J., Piekorz, R., Reichert, A., Volleth, M., Wolf, M.J., Gödecke, A., Cyganek, L., Gelb, B.D., Tartaglia, M., Schmitt, J., Krüger, M., Zenker, M., Kensah, G., Ahmadian, M.R.. “Molecular and cellular evidence for the impact of a hypertrophy-associated RAF1 variant on the structure and function of contractile machinery in bioartificial cardiac tissues”. Communications Biology (2023).

  3. Nau, J., Bazjir, F., Nakhaei-Rad, S., Amin, E., Wolf, M.J., Saucerman, J.J., Lorenz, K. and Ahmadian, M.R. The microenvironment of the pathogenesis of cardiac hypertrophy (2023) Cells Jul 4;12(13):1780. doi: 10.3390/cells12131780.

  4. Shah, S.A., Reagan, C.E., Billcheck, H.O., Bradley, L.A., French, B.A., Isakson, B.E., Wolf, M.J., and Epstein, F.H. Role of iNOS in preventing and reversing coronary microvascular disease due to a high-fat high-sucrose diet. JACC: Basic to Translational Science (2023) https://doi.org/10.1016/j.jacbts.2022.11.005

  5. Julie A. Fischer, J.A., Monroe, T.O., Lorenzo, L.P., Sawicki, K.T., Mattia Quattrocelli, M., Bauer, R., Kearns, S.D., Wolf, M.J., Puckelwartz, M.J., McNally, E.M. Opposing effects of genetic variation in MTCH2 for obesity versus heart failure. Human Molecular Genetics (2023) Jan 1;32(1):15-29. doi: 10.1093/hmg/ddac176. https://doi.org/10.1093/hmg/ddac176

  6. Shah, S.A., Echols, J.T., Wolf, M.J., and Epstein, F.H. Accelerated fatty acid composition MRI of epicardial adipose tissue: development and application to eplerenone treatment in a mouse model of obesity-induced coronary microvascular disease. Magnetic Resonance in Medicine (2022) 1–14. https://doi.org/10.1002/mrm.29348

  7. Young, A. Bradley, L.A., Farrar, E., Bilcheck, H.O., Tkachenko, S., Saucerman, J.J., Bekiranov, S., and Wolf, M.J. Inhibition of DYRK 1a Enhances Cardiomyocyte Cycling after Myocardial Infarction. Circ Res. (2022); 130:1345–1361

  8. Drake, J.C., Wilson, R.J., Laker, R.C., Guan, Y., Spaulding, H.R., Nichenko, A.S., Shen, W., Shang, H., Dorn, M.V. Huang, K., Zhang, M., Bandara, A.B., Brisendine, M.H, Kashatus, J.A., Sharma, P.R., Young, A., Gautam, J., Cao, R., Wallrabe, H., Chang, P.A., Wong, M., Desjardins, E.M., Hawley, S.A., Christ, G.J., Kashatus, D.F., Miller, C.L., Wolf, M.J., Periasamy, A., Steinberg, G.R., Hardie, D.G., and Yan, Z. Mitochondria-localized AMPK responds to local energetics and plays a role in exercise and energetic stress-induced mitophagy. (2021) Proc. Nat. Acad. Sci. U.S.A. 118 (37) e2025932118:

  9. Good, M.E.*, Young, A., Wolpe, A.G., Johnstone, S.R., Hall, P.J., Duffy, C.K., Blanton, R.M., Leitinger, N. Wolf, M.J.*, and Isakson, B.E.* Endothelial pannexin 1 contributes to impaired cardiac function following myocardial infarction. (2021) Circ. Res.

  10. Bradley, L.A., Young, A., Li, H. Billcheck, H.O. and Wolf, M.J.* (2021) Loss of Endogenously Cycling Adult Cardiomyocytes Worsens Myocardial Function. Circ. Res. 128 (2):155-168

  11. Petersen, C.E., Wolf, M.J., and Smyth, J.T. (2020) Suppression of store-operated calcium entry causes dilated cardiomyopathy of the Drosophila heart. Open Biology 9, bio049999.

  12. Cresci, S., Pereira, N.L., Ahmad, F., Byku, M., de las Fuentes, L., Lanfear, D.E., Reilly, C.M., Owens, A.T., and Wolf, M.J. (2019) AHA Scientific Statement Heart Failure in the Era of Precision Medicine. A Scientific Statement from the American Heart Association, Circulation: Genomics and Precision Medicine. 12(10):458-485.

  13. Woo, L.A., Tkachenko, S., Ding, M.; Plowright, A.T., Engkvist, O., Andersson, E.; Drowley, L., Barrett, I., Firth, I., Akerblad, P., Wolf, M.J., Bekiranov, S. ; David L Brautigan, D.L., ; Wang, Q.D., Saucerman, J.; (2019) High-content phenotypic screen for compounds that induce proliferation of human iPSC-derived cardiomyocytes reveals L-type calcium channels as targets J. Mol. and Cell. Card. 127:204-214

  14. Abraham, D.M., Lee, T.E., Watson, L.J., Mao, L., Chandok, G., Wang, H.G., Frangakis, S., Pitt, G.S., Shah,S.H., Wolf, M.J. and Rockman, H.A. The 2-pore domain potassium channel TREK-1 mediates cardiac fibrosis and diastolic dysfunction. J Clin Invest. (2018-in press)

  15. Wolf, M.J.*, Noeth, D., Rammohan, C., and Shah, S.H. (2016) Complexities of Genetic Testing in Familial Dilated Cardiomyopathy. Circ. Cardiovasc Genet. 9:95-99.

  16. Gao QQ, Wyatt E, Goldstein JA, LoPresti P, Castillo LM, Gazda A, Petrossian N, Earley JU, Hadhazy M, Barefield DY, Demonbreun AR, Bönnemann C, Wolf M, McNally EM.* (2015) Reengineering a transmembrane protein to treat muscular dystrophy using exon skipping. J Clin Invest. 125(11):4186-95.

  17. Hardy, C., Birse, R., Wolf, M.J., Yu, L., Bodmer, R. and Gibbs, A.* (2015) Obesity-associated Cardiac Dysfunction in Starvation-Selected Drosophila melanogaster. The American Journal of Physiology Regulatory, Integrative and Comparative Physiology.

  18. Yu, L., Daniels, J.P., Wu, H., and Wolf, M.J.* (2015) Cardiac hypertrophy induced by active Raf depends on Yorkie-mediated transcription. Science Signaling. 8(362):ra13

  19. Lee, T.E., Yu, L., Wolf, M.J., Rockman, H.A.* (2014) Galactokinase is a novel modifier of calcineurin-induced cardiomyopathy in Drosophila. Genetics. 198(2):591-603.

  20. Abraham, D.M. and Wolf, M.J.* (2013) Disruption of Sarcoendoplasmic Reticulum Calcium ATPase Function in Drosophila Leads to Cardiac Dysfunction. PLoS ONE 8(10): e77785. doi:10.1371/journal.pone.0077785

  21. Yu, L., Daniels, J., Glaser, A. and Wolf M.J.* (2013) Raf-mediated Cardiac Hypertrophy in Adult Drosophila. Disease Models and Mechanisms, Apr 4. [Epub ahead of print]

  22. Wooten, E.C., Bartleson Hebl, V., Wolf, M.J., Greytak, S.R., Orr, N., Draper, I., Calvino, J.E., Kapur, N.K., Maron, M.S., Kullo, I.J., Ommen, S.R., Bos, J. M., Ackerman,M.J., Huggins, G.S.* (2013) Formin Homology 2 Domain Containing 3 (FHOD3) Variants Associated with Hypertrophic Cardiomyopathy. Circ Cardiovasc Genet. 6: 10-18.

  23. Casad, M.E., Yu L., Daniels, J.P., Wolf, M.J., Rockman, H.A.* (2012) Deletion of Siah-interacting Protein Gene in Drosophila Causes Cardiomyopathy. Mol Genet Genomics. 287(4):351-60.

  24. Casad, M.E., Abraham, D., Kim, I.M., Frangakis, S., Dong, B., Lin, N., Wolf, M.J., Rockman, H.A.* (2011) Cardiomyopathy is Associated with Ribosomal Protein Gene Haplo-insufficiency in Drosophila melanogaster. Genetics. 2011 Nov;189(3):861-70.

  25. Lin, N., Badie, N., Yu, L., Abraham, D., Cheng, H., Bursac, N., Rockman, H.A., and Wolf, M.J.* (2011) A Method to Measure Myocardial Calcium Handling in Adult Drosophila. Circ. Res. May 27;108(11):1306-15.

  26. Goldstein, J.A., Kelly, S.M., Lopresti, P.P., Heydemann, A., Earley, J.U., Ferguson, E.L., Wolf, M.J., McNally, E.M.* (2010) SMAD Signaling Drives Heart and Muscle Dysfunction in a Drosophila Model of Muscular Dystrophy. Hum. Mol. Genet. 20(5):894-904.

  27. Yu, L., Lee, T. Lin, N., and Wolf, M.J.* (2010) Affecting Rhomboid-3 Function Cause a Dilated Heart in Adult Drosophila. PLoS Genetics 6(5):e1000969.

  28. Kim, IM., Wolf, M.J., and Rockman, H.A.* (2010) Gene Deletion Screen for Cardiomyopathy in Adult Drosophila Identifies a New Notch Ligand. Circ. Res. 106(7):1233-43. PMC2860286

  29. Kim,Il and Wolf, M.J.* (2009) Serial Examination of an Inducible and Reversible Dilated Cardiomyopathy in Individual Adult Drosophila. PLoS One. Sep 22;4(9):e713 PMC2740824.

  30. Yoo, B., Lemaire, A., Mangmool, S., Wolf, M.J., Curcio, A., Mao, L., Rockman, H.A.* (2009) Beta1-adrenergic Receptors Stimulate Cardiac Contractility and CaMKII Activation In Vivo and Enhance Cardiac Dysfunction Following Myocardial Infarction. Am J Physiol Heart Circ Physiol. 297(4):H1377-86. PMC2770777

  31. Allikian, M.J., Bhabha, G., Dospoy, P., Heydemann, A., Ryder, P., Earley, J.U., Wolf, M.J., Howard A. Rockman, H.A., and McNally, E.M.* (2007) Reduced Lifespan with Heart and Muscle Dysfunction in Drosophila sarcoglycan mutants. Hum. Mol. Genet. 16: 2933-43.

  32. Curcio, A., Noma, T., Naga Prasad, S.V., Wolf, M.J., Lemaire, A., Perrino, C., Mao, L., Rockman, H.A.* (2006) Competitive Displacement of Phosphoinositide-3 kinase from Beta-adrenergic Receptor Kinase-1 Improves Post-infarction Adverse Myocardial Remodeling. Am. J. Physiol. Heart. Circ. Physiol. 291(4):H1754-60.

  33. Volovyk, Z.M., Wolf, M.J., Prasad, S.V., Rockman, H.A.* (2006) Agonist-stimulated Beta-adrenergic Receptor Internalization Requires Dynamic Cytoskeletal Actin Turnover. J. Biol. Chem. 281: 9773-80.

  34. Wheeler, F.C., Fernandez, L., Carlson, K.M., Wolf, M.J., Rockman, H.A., Marchuk, D.A.* (2006) QTL Mapping in a Mouse Model of Cardiomyopathy Reveals an Ancestral Modifier Allele Affecting Heart Function and Survival. Mamm. Genome. 16:414-23.

  35. Wolf, M.J., Amerin, H., Izatt, J.A., Choma, M.A., Reedy, M.C., and Rockman, H.A.* (2006) Drosophila as a Model for the Identification of Genes Causing Adult Human Heart Disease. Proc. Natl. Acad. Sci. U.S.A. 103: 1394-9

  36. Perrino, C., Naga Prasad, S.V., Patel, M., Wolf, M.J., and Rockman, H.A.* (2005) Targeted Inhibition of Beta-Adrenergic Receptor Kinase-1–Associated Phosphoinositide-3 Kinase Activity Preserves Beta-Adrenergic Receptor Signaling and Prolongs Survival in Heart Failure Induced by Calsequestrin Overexpression. J. Am. Coll. Cardiol. 45:1862-70.

  37. Jenkins, CM, Wolf, M.J., Mancuso, DJ, Gross, RW* (2001) Identification of the Calmodulin-binding Domain of Recombinant Calcium-independent Phospholipase A2beta. J. Biol. Chem. 276: 7129-7135.

  38. Ramanadham, S., Wolf, M.J., Li, B, Boher, A, Turk, J.* (1997) Glucose-Responsivity and Expression of an ATP-Stimulatable, Calcium-Independent Phospholipase A2 Enzyme in Clonal Insulinoma Cell Lines. Biochim. Biophys. Acta 1344: 153-164.

  39. Wolf, M.J., Wang, J. Turk, J. Gross, R.W.* (1997) Depletion of Intracellular Calcium Stores Activates Smooth Muscle Cell Calcium-independent Phospholipase A2. J. Biol. Chem. 272: 1522-1526.

  40. Ramanadham, S., Wolf, M.J., Ma, Z., Li, B. Wang, J., Gross, R.W., Turk, J.* (1996) Evidence for Association of an ATP-Stimulatable Calcium-Independent Phospholipase A2 from Pancreatic Islets and HIT Insulinoma Cells with a Phosphofructokinase-like Protein. Biochem. 35: 5364-5471.

  41. Wolf, M.J. and Gross, R.W.* (1996) Expression, Purification, and Kinetic Characterization of a Recombinant 80-kDa Intracellular Calcium-independent Phospholipase A2. J. Biol. Chem. 217: 30879-30885.

  42. Wolf, M.J. and Gross, R.W.* (1996) The Calcium-Dependent Association and Functional Coupling of Calmodulin with Myocardial Phospholipase A2. J. Biol. Chem. 217: 20989-20992.

  43. Wolf, M.J., Izumi, Y. Zorumski, C.F., Gross, R.W.* (1995) Long-term Potentiation Requires Activation of Calcium-Independent Phospholipase A2. FEBS Lett. 377: 358-362.

  44. Gross, R.W.*, Rudolph. A.E., Wang, J. Sommers, C.D., Wolf, M.J. (1995) Nitric Oxide Activates the Glucose-Dependent Mobilization of Arachidonic Acid in a Macrophage-Like Cell Line (RAW264.7) That Is Largely Mediated by Calcium-Independent Phospholipase A2. J. Biol. Chem. 270: 14855-14858.

  45. Ramanadham, S., Wolf, M.J., Jett, P.A., Gross, R.W., Turk, J.* (1994) Characterization of an ATP-stimulatable Calcium-Independent Phospholipase A2 from Clonal Insulin-Secreting HIT Cells and Rat Pancreatic Islets. Biochem. 33: 7442-7452.

  46. Hazen, S.L., Wolf, M.J., Ford, D.A., Gross, R.W.* (1994) The Rapid and Reversible Association of Phosphofructokinase with Myocardial Membranes During Myocardial Ischemia. FEBS Lett. 339: 213-21.

    Invited reviews in peer-reviewed journals

  1. Young, A., Bradley, L.A., and Wolf, M.J. “In vivo methods to monitor cardiomyocyte proliferation” J. Cardiovasc. Dev. Dis. 2022, 9, 73. https://doi.org/10.3390/jcdd9030073.

  2. Wolf, M.J.* "HETE"ing up mitochondria in human heart failure. J. Biol. Chem (2018); 293 (1): 130-131. (Invited Editorial)

  3. Wolf, M.J.* (2016) A SPARCling Study of Drosophila Cardiomyopathy. Circ. Cardiovasc Genet. 9:104-106 (Invited Editorial)

  4. Wolf, M.J.* (2013) Towards understanding the impact of sarcomeric gene mutations. JACC Heart Fail. 1(6):467-8

  5. Wolf, M.J.* (2012) Modeling Cardiomyopathies in Drosophila. Trends in Cardiovascular Medicine 22(3):55-61. (Invited Review)

  6. Wolf M.J.*, Rockman HA. (2011) Drosophila, Genetic Screens, and Cardiac Function. Circ. Res. Sep 16;109(7):794-806.

  7. Wolf, M.J.* and Rockman, H.A. (2009) Drosophila Melanogaster as a Model System for the Genetics of Postnatal Cardiac Function. Drug Discovery Today: Disease Models 1;5(3):117-123. PMC2753975 (Invited Review)

    Invited book chapters

  1. Yu, L., Daniels, J.P., and Wolf, M.J. (2014) “Vascular and Cardiac Studies in Drosophila”. Manual of Cardiovascular Techniques, Wiley Press.

  2. Wolf, M.J., Takibana, H. And Rockman, H.A. (2005) “Methods for the Detection of Altered Beta-Adrenergic Signaling Pathways in Hypertrophied Hearts”. Molecular Cardiology, Humana Press.

    Abstracts, oral presentations

  1. Bradley, L.A., Young, A., Li, H. Billcheck, H.O. and Wolf, M.J. A Transgenic Approach Using Dual Orthologous DNA Recombinases To Quantify Subpopulations Of Cycling Cardiomyocytes in Vivo. American Heart Association Basic Cardiovascular Sciences Conference 2020.

  2. Young, A., Bradley, L.A., Li, H. Billcheck, H.O. and Wolf, M.J. Inhibition Of Dual-specificity Tyrosine Phosphorylation-regulated Kinase 1a Attenuates Left Ventricular Dysfunction After Myocardial Infarction. American Heart Association Basic Cardiovascular Sciences Conference 2020.

  3. Fischer, J.A., Quattrocelli, M., Puckelwartz, M., Wolf, M.J., and McNally, E.M. Evaluating MTCH2 as a Modifier of Cardiomyopathy. American Heart Association Basic Cardiovascular Sciences Conference 2020.

  4. Auger, D.A., Roy, R.J., Tonleu, F.A., Bradley, L., Good, M.E., Isakson, B.E., Bamberg, K., Johansson, U., Akerblad, P., Epstein, F.H., Wolf, M.J. Eplerenone, a mineralocorticoid receptor antagonist, protects the microvasculature and prevents impaired myocardial perfusion reserve in mice fed a high fat diet.* Society for Cardiovascular Magnetic Resonance 20th Annual Scientific Sessions (2017)

  5. Patterson S.C., Howard Rockman, H.A., and Wolf M.J. SMYD-mediated histone methylation as a mechanism governing cardiomyocyte growth* Howard Hughes Medical Institute Medical (HHMI) Research Fellows Program (5/26/2015)

    Dr. Patterson was a medical student who received Howard Hughes Medical Institute Medical (HHMI) Research Fellowship and I was her mentor.

  6. Hardy, C., Birse, R., Wolf, M.J., Gibbs, A. Obesity-induced Cardiac Dysfunction in Starvation-Selected Drosophila.* 52nd Annual Drosophila Research Conference, Chicago IL (3/7/2015)

  7. Abraham, D., Lee, T., Watson, L., Mao, L., Chandok, G., Wolf, M.J., Rockman, H.A. TREK-1 Modulates Fibrosis & Diastolic Dysfunction Through Activation of Stress-Activated Kinases*. Melvin L. Marcus Young Investigator Award in Cardiovascular Sciences Finalists (2014) American Heart Association Scientific Sessions.

  8. Wolf, M.J. Drosophila Arrhythmias*, Heart Rhythm Society of America, Denver, Colorado (5/2013)

  9. Wolf, M.J. Drosophila screen to identify new heart failure genes* International Society for Heart Research. Philadelphia, PA. (2011)

  10. Wolf, M.J. Modeling the Complexity of heart Failure in Drosophila* American Heart Association Meeting, Orlando, FL. (11/2011)

  11. Yu, L., Daniels, J.P. and Wolf, M.J. Receptor Tyrosine Kinase Signaling Causes Cardiac Hypertrophy in Adult Drosophila* American Heart Association Meeting, Orlando, FL. (11/2011)

  12. Wolf, M.J. Modeling the Complexity of heart Failure in Drosophila* American Heart Association Meeting, Orlando, FL. (11/2011)

  13. Yu, L., Lee, T. Lin, N., and Wolf, M.J. Alterations in Rhomboid-3 cause a dilated heart in adult Drosophila. American Society of Genetics: Model Organisms to Human Biology, Boston MA. (2010)

  14. Goldstein, J.A., Kelly, S.M., LoPresti, P.P., Heydemann, A., Early, J.U., Wolf, M.J., McNally, E.M. TGF beta Signaling Drives Progression Cardiomyopathy and Muscular Dystrophy. American Heart Association Scientific Sessions Meeting (11/2010)

  15. Kim, I.M., Wolf, M.J., Rockman, H.A. Gene Deletion Screen for Cardiomyopathy in Adult Drosophila Identifies Weary (Wry): A New Notch Ligand.* American Heart Association Scientific Sessions Meeting (11/2009)

  16. Wolf, M.J. Deficiency in Rhomboid-3 Causes a Dilated Cardiomyopathy in Adult Drosophila* American Heart Association Meetings, Orlando, FL. (11/2009)

  17. Wolf, M.J. Heart Failure Genes in Drosophila* Heart Failure Society of America 12th Annual Scientific Meeting, Toronto, Canada (2008)

  18. Wolf, M.J. High Throughput Screening for Cardiovascular Genetic Defects. American Heart Association Meetings, New Orleans, LA. (11/2008)

  19. Kim, I.M., Casad, M.E., Wolf, M.J., Rockman, H.A. Gene deletion screen in adult Drosophila identifies possible candidate gene for dilated cardiomyopathy.* American Heart Association Scientific Sessions Meeting (11/2007)

  20. Wolf, M.J., Amerin, H., Izatt, J.A., Choma, M.A., Reedy, M.C., Rockman, H.A. Genome-wide screen in adult Drosophila to identify novel cardiomyopathy genes* Louis N. and Arnold M. Katz Basic Science Research Prize for Young (11/2005)

    Investigators Finalists, American Heart Association Scientific Sessions, Dallas, TX.

  21. Lemaire, A., Curcio, A., Prasad, S.V.N., Noma, T., Wolf, M.J., Kobilka, B.K., Rockman, H.A. beta(1)-Adrenergic receptors selectively induce Ca2+-calmodulin-dependent protein kinase II activity in the development of heart failure after myocardial infarction* American Heart Association Scientific Sessions Meeting (11/2005)

  22. Curcio, A., Lemaire, A., Wolf, M.J., Perrino, C., Mao, L., Prasad, S.V.N., Rockman, H.A. Displacement of phosphoinositide 3-kinase from beta adrenergic receptor kinase 1 preserves cardiac function after myocardial infarction. American Heart Association Scientific Sessions Meeting (11/2005)

  23. Wolf, M.J., Volovky, Z., Rockman, H.A. Beta-Adrenergic Receptor Signaling is Modulated by Cofilin, a Regulator of Cytoskeletal Dynamics* American Heart Association Scientific Sessions (11/2004)

  24. Donahue, M.P., Wolf, M.J., Jennings, J.L., Pedersen, B., Hauser, E.R., Marchuk, D.A., and Kraus, W.E. PPARI7 Polymorphism is Associated with Reduced Cardiac Function Identifying it as a Modifier of Heart Failure* American Heart Association Scientific Sessions (11/2004)

  25. Lemaire, A., Curcio, A., Noma, T. Wolf, M.J., Kobilka, B.K., and Rockman, H.A. beta(2)-Adrenergic receptors selectively prevent in vivo murine heart failure* American Heart Association Scientific Sessions Meeting (11/2004)