Michael B. Lawrence

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Primary Appointment

Associate Professor, Biomedical Engineering

Education

  • BA, Chemistry, Rice University
  • PhD, Chemical Engineering, Rice University
  • Postdoc, Immunology, Harvard University

Research Disciplines

Biophysics, Biotechnology, Cardiovascular Biology, Cardiovascular Biology

Research Interests

Vascular and Molecular Engineering

Research Description

Our laboratory is developing applications of molecular mechanics to the challenges of targeted drug and gene delivery. To increase specificity and reduce toxic side effects, we are incorporating leukocyte adhesion receptors into liposomes and polymeric microparticles containing encapsulated therapeutics to target defective cells with high specificity. To this end, we are using a mutagenesis approach coupled with biophysical analysis of mechanically stressed bonds to identify the controlling features of adhesion receptors that permit binding in the presence of fluid shear forces.
Additionally, we have developed several model flow systems to examine the dynamics of platelet-leukocyte-vessel wall interactions that take place during the acute phases of an inflammatory challenge or the formation of blood clots on the vessel wall. Common to interactions between platelets, leukocytes, and the vessel wall is the involvement of a family of receptors called selectins that support transient adhesions in which leukocytes and platelets roll along the vessel wall in shear flow. These studies have significance for the understanding of the regulatory effects of blood flow, adhesion receptor expression, and chemokines on inflammatory responses and blood clotting.

Personal Statement

Our laboratory is developing applications of molecular mechanics to the challenges of targeted drug and gene delivery. To increase specificity and reduce toxic side effects, we are incorporating leukocyte adhesion receptors into liposomes and polymeric microparticles containing encapsulated therapeutics to target defective cells with high specificity. To this end, we are using a mutagenesis approach coupled with biophysical analysis of mechanically stressed bonds to identify the controlling features of adhesion receptors that permit binding in the presence of fluid shear forces.
Additionally, we have developed several model flow systems to examine the dynamics of platelet-leukocyte-vessel wall interactions that take place during the acute phases of an inflammatory challenge or the formation of blood clots on the vessel wall. Common to interactions between platelets, leukocytes, and the vessel wall is the involvement of a family of receptors called selectins that support transient adhesions in which leukocytes and platelets roll along the vessel wall in shear flow. These studies have significance for the understanding of the regulatory effects of blood flow, adhesion receptor expression, and chemokines on inflammatory responses and blood clotting.

Selected Publications

Wang CW, Perez MJ, Helmke BP, Viola F, Lawrence MB, Integration of acoustic radiation force and optical imaging for blood plasma clot stiffness measurement., 2015; PloS one. 10(6) e0128799. PMID: 26042775 | PMCID: PMC4456080

Tlaxca JL, Rychak JJ, Ernst PB, Konkalmatt PR, Shevchenko TI, Pizarro TT, Pizzaro TT, Rivera-Nieves J, Klibanov AL, Lawrence MB, Ultrasound-based molecular imaging and specific gene delivery to mesenteric vasculature by endothelial adhesion molecule targeted microbubbles in a mouse model of Crohn's disease., 2012; Journal of controlled release : official journal of the Controlled Release Society. 165(3) 216-25. PMID: 23142578 | PMCID: PMC3711027

Mauldin FW, Viola F, Hamer TC, Ahmed EM, Crawford SB, Haverstick DM, Lawrence MB, Walker WF, Adaptive force sonorheometry for assessment of whole blood coagulation., 2010; Clinica chimica acta; international journal of clinical chemistry. 411(9) 638-44. PMID: 20096680 | PMCID: PMC2911630

Bailey AM, Lawrence MB, Shang H, Katz AJ, Peirce SM, Agent-based model of therapeutic adipose-derived stromal cell trafficking during ischemia predicts ability to roll on P-selectin., 2009; PLoS computational biology. 5(2) e1000294. PMID: 19247427 | PMCID: PMC2636895

Paschall CD, Lawrence MB, L-selectin shear thresholding modulates leukocyte secondary capture., 2008; Annals of biomedical engineering. 36(4) 622-31. PMID: 18299990 | PMCID: PMC3711019

Schmidt BJ, Huang P, Breuer KS, Lawrence MB, Catch strip assay for the relative assessment of two-dimensional protein association kinetics., 2008; Analytical chemistry. 80(4) 944-50. PMID: 18217724 | PMCID: PMC3335339

Paschall CD, Guilford WH, Lawrence MB, Enhancement of L-selectin, but not P-selectin, bond formation frequency by convective flow., 2007; Biophysical journal. 94(3) 1034-45. PMID: 17890384 | PMCID: PMC2186251

Ham AS, Goetz DJ, Klibanov AL, Lawrence MB, Microparticle adhesive dynamics and rolling mediated by selectin-specific antibodies under flow., 2006; Biotechnology and bioengineering. 96(3) 596-607. PMID: 16917925 | PMCID: PMC3711028

Kadash KE, Lawrence MB, Diamond SL, Neutrophil string formation: hydrodynamic thresholding and cellular deformation during cell collisions., 2004; Biophysical journal. 86(6) 4030-9. PMID: 15189898 | PMCID: PMC1304303

Rinko LJ, Lawrence MB, Guilford WH, The molecular mechanics of P- and L-selectin lectin domains binding to PSGL-1., 2003; Biophysical journal. 86(1) 544-54. PMID: 14695299 | PMCID: PMC1303823