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Immunohistochemistry Facility Services

We are located in MR5 on the ground floor in room G207.

Please see The Rules Of Use For Our Facility but all services must provide a PTAO at time of request.


Histological Sectioning- This service is to section frozen and paraffin samples onto slides for routine and special staining techniques. We are not a certified BSL2 facility, therefore no sectioning of human samples is allowed. IF human samples have been previously fixes in paraffin then we can allow sectioning and processing.
1. Paraffin: Special Instructions should be written down
  • Orientation of sample
  • How many sections per slide, thickness of section, number of slides to section
  • Area to start collecting samples
  • 2. Frozen: Tissue must be brought to lab embedded in OCT Compound and special Instructions should be written down

  • Orientation of sample
  • How many sections per slide, thickness of section, number of slides to section
  • Area to start collecting samples
  • Supplies: Must provide slides (Superfrost Plus), slide boxes and blades
    NOTE: We are not a certified BSL2 facility, therefore no sectioning of human samples is allowed. IF human samples have been previously fixed in paraffin, then we can allow sectioning and processing.


    Paraffin Embedding- This service is to embed samples in paraffin for sectioning.
    1. Tissue must be brought to the lab in pencil labeled cassettes in 70% ethanol
    2. Special Instructions should be written down

    Adipocyte Cell Measuring- This service is to measure cell sizing in adipocyte cells.
    1. Pictures should be taken at 10x 1360x1024 and burned onto a cd
    2. Special Instructions should be written down
  • Number of sections that need to be analyzed
  • Number of cells that need to be measured per section

  • Fixation- Fixation time is based on the type of tissue, size of tissue, fixation method and type of fixation. Please call ahead to discuss.

    Training- General equipment usage (microtome’s, water baths, etc) and training are provided as a free service to our resident CVRC labs. For a nominal labor charge for staff time, we do provide training to our member labs and/or more advanced training of histological sectioning as well. Once trained and approved; staff has access to schedule and use equipment and facilities without supervision; provided they follow rules and regulations established below. We ask that all labs bring their own supplies but if you utilize our setups and/or materials, we will ask that they be replenished based on your usage.

    Tissue Processing- Contact Histology Staff to discuss your needs and time that will be needed.

    Immunohistochemistry (IHC) (single)- Contact Histology Staff to discuss your needs and time that will be needed.

    Immunoflourescence (IF) (single, double, triple)- Contact Histology Staff to discuss your needs and time that will be needed.

    Hematoxylin & Eosin- Contact Histology Staff to discuss your needs and time that will be needed.

    Luna’s Method for Iron- Contact Histology Staff to discuss your needs and time that will be needed.

    Modified Russell-Movat Pentachrome Method- Contact Histology Staff to discuss your needs and time that will be needed.

    Oil Red O on Frozen Sections- Contact Histology Staff to discuss your needs and time that will be needed.

    Perl’s Method for Iron- Contact Histology Staff to discuss your needs and time that will be needed.

    Picrsirius Red Method for Collagen- Contact Histology Staff to discuss your needs and time that will be needed.

    Measuring of Fat Cells using Scion Imagine- Contact Histology Staff to discuss your needs and time that will be needed.

    Related Links:

    Histology Main Page
    The Rules Of Use For Our Facility
    How To Schedule A Service

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    Contact Us

    Robert M. Berne Cardiovascular Research Center
    University of Virginia- School of Medicine
    415 Lane Rd, Suite 1010
    PO Box 801394
    Charlottesville, VA 22908
    Phone: (434) 924-5500
    Fax: (434) 924-2828
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