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Immunohistochemistry Facility Services

MR5 Room 1238

Histological Sectioning- This service is to section frozen and paraffin samples onto slides for routine and special staining techniques.
1. Paraffin: Special Instructions should be written down
  • Orientation of sample
  • How many sections per slide, thickness of section, number of slides to section
  • Area to start collecting samples

    • 2. Frozen: Tissue must be brought to lab embedded in OCT Compound and special Instructions should be written down

  • Orientation of sample
  • How many sections per slide, thickness of section, number of slides to section
  • Area to start collecting samples

    • Supplies: Must provide slides (Superfrost Plus), slide boxes and blades

    Paraffin Embedding- This service is to embed samples in paraffin for sectioning.
    1. Tissue must be brought to the lab in pencil labeled cassettes in 70% ethanol
    2. Special Instructions should be written down
    Adipocyte Cell Measuring- This service is to measure cell sizing in adipocyte cells.
    1. Pictures should be taken at 10x 1360x1024 and burned onto a cd
    2. Special Instructions should be written down
  • Number of sections that need to be analyzed
  • Number of cells that need to be measured per section
    • Fixation- Fixation time is based on the type of tissue, size of tissue, fixation method and type of fixation. Please call ahead to discuss.
    Training- General equipment usage (microtome’s, water baths, etc) and training are provided as a free service to our CVRC labs. More advanced training of histological sectioning can be provided for a nominal labor charge for staff time. Once trained and approved; staff has access to schedule and use equipment and facilities without supervision; provided they follow rules and regulations established below. We ask that all labs bring their own supplies but if you utilize our setups and/or materials, we will ask that they be replenished based on your usage.
    Tissue Processing- Contact Histology Staff to discuss your needs and time that will be needed.
    Immunohistochemistry (IHC) (single)- Contact Histology Staff to discuss your needs and time that will be needed.
    Immunoflourescence (IF) (single, double, triple)- Contact Histology Staff to discuss your needs and time that will be needed.
    Hematoxylin & Eosin- Contact Histology Staff to discuss your needs and time that will be needed.
    Luna’s Method for Iron- Contact Histology Staff to discuss your needs and time that will be needed.
    Modified Russell-Movat Pentachrome Method- Contact Histology Staff to discuss your needs and time that will be needed.
    Oil Red O on Frozen Sections- Contact Histology Staff to discuss your needs and time that will be needed.
    Perl’s Method for Iron- Contact Histology Staff to discuss your needs and time that will be needed.
    Picrsirius Red Method for Collagen- Contact Histology Staff to discuss your needs and time that will be needed.
    Measuring of Fat Cells using Scion Imagine- Contact Histology Staff to discuss your needs and time that will be needed.