Animal Experiments

Genotyping Protocols

Ligation Injury

Pluronic Gel Application to Rat Carotid Arteries

Western Diet 88137

Cell Culture

Culture Experiment Cholesterol Treatment

Mouse Primary SMC

Oligofectamine 6-well

SMC Culture Methods

SMC Migration Assay

Trizol RNA Extraction

Aortic Explant Assay

Gelatin Coated Slides Peirce-Cottler Lab

Isolation of Bone Marrow Derived Macrophages

Katie SMC Condition Media Protocol

Flow Cytometry

Flow Digestion Buffer

Flow Experiment Design Calculations

M1 vs M2 Flow Cytometry

New Flow Protocol

High-throughput flow panel for SVF

Image Analysis

BCA IF Confocal Data Analysis Protocol

Immunostaining Analysis Using ImagePro

Staining Protocols

Modified Russell-Movat's Stain

Protocol for Staining OCT4 YFP SMaA DAPI

Protocol for Staining YFP SMaA Mac-2

Singleparaffin Section IHC sheet Oct4 C10

Sudan IV Stain

Whole Mounting Adipose Tissue

Tissue Harvesting Processing

Aortic Sinus Sectioning Mouse

BCA Sectioning Mouse

Carotid Sectioning Post-ligation Mouse

Harvesting of Tissue from Ligation Injured Mice

PLP Fixation from ESG

Tissue Harvesting for Spontaneous Atherosclerosis

Western Blot

Western Blot Protocol

Western Blotting Conditions

Other Protocols and Reagents

ISH-PLA Owens Lab Protocol

Key Biologicals and Chemical Resources

MicroChiP Protocol DG